Cytotoxicities of Antibacterial Silane Coupling Agents

• MIYAKE Kaori

  Division of Restorative Dentistry, Department of Oral Medicine, Kanagawa Dental College

• OHASHI Katsura

  Division of Restorative Dentistry, Department of Oral Medicine, Kanagawa Dental College

• NIHEI Tomotaro

  Division of Restorative Dentistry, Department of Oral Medicine, Kanagawa Dental College

• SHIMIZU Tota

  Division of Prosthetics, Department of Oral and Maxillofacial Rehabilitation, Kanagawa Dental College

• YAMAGUCHI Shinichiro

  Department of Comprehensive Dentistry, Kanagawa Dental College

• KONDO Yukishige

  Department of Industrial Chemistry, Tokyo University of Science

• YOSHINO Norio

  Department of Industrial Chemistry, Tokyo University of Science

• TERANAKA Toshio

  Division of Restorative Dentistry, Department of Oral Medicine, Kanagawa Dental College

Bibliographic Information

Other Title

• 抗菌性シランカップリング剤の細胞毒性

Abstract

We have considered that decreasing the surface free energy of the tooth surface may help prevent caries and periodontal disease. Recently, we successfully synthesized two antibacterial silane coupling agents, N-allyl-N-decyl-N-methyl-N-trimethoxysilylpropylammonium iodide (10-I) and N-allyl-N-methyl-N-trimethoxysilylpropyl-N-octadecylammonium iodide (18-I). The aim of this study was to investigate the cytotoxicities of these modifier agents. Glass plates were dipped in 20 mmol/l of 10-I and 18-I solutions for 1 hour at 20℃, and then were sterilized with ethylene oxide gas for 24 hours at 35℃. The modified glass plates were immersed in cell culture medium (MO5) for 24 hours at 37℃ to examine the toxicity of the extraction procedure. The extracted medium (100%) was diluted with the MO5 into ten different concentrations (0.5-50%). 0.5 ml of the V79 cell suspension (100 cells/ml) was seeded on a 24-well cell culture plate and pre-incubated for 6 hours at 37℃ in a 5% CO₂ atmosphere. After removal of MO5, 0.5 ml of the extracts containing MO5 was poured onto the cell culture of V79 cells. After 6-day cultivation, cells were stained with 0.1% methylene blue solution, and calculated. The cytotoxicities of various modified agents were evaluated according to the 50% inhibitory concentration (IC₅₀). The IC₅₀ value of 18-I was 18.8%, while that of 10-I was more than 100%. These results suggest that 10-I has no cytotoxicity, whereas 18-I has moderate cytotoxicity.

Journal

• The Japanese Journal of Conservative Dentistry

  The Japanese Journal of Conservative Dentistry 54 (6), 393-398, 2011

  The Japanese Society of Conservative Dentistry

Keywords

• Cytotoxicity
• Antibacterial
• Silane coupling agent

Details

• CRID: 1390282680496948480
• NII Article ID: 110009419167
• NII Book ID: AN00191201
• DOI: 10.11471/shikahozon.54.393
• ISSN: 21880808
• ISSN: 03872343
• Web Site: https://search.jamas.or.jp/link/ui/2012141591
• Text Lang: ja
• Data Source:
  • JaLC
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed