A Novel Method for Disinfection of Root Canal System by Enhanced Delivery of Medicaments using Ultrasound with Nanobubbles
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- EBA Hisanori
- Center of Advanced Medicine for Dental and Oral Diseases, National Center for Geriatrics and Gerontology Research Institute:Department of Endodontics, School of Dentistry, Aichi-Gakuin University
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- IOHARA Koichiro
- Center of Advanced Medicine for Dental and Oral Diseases, National Center for Geriatrics and Gerontology Research Institute
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- TACHIBANA Katsuro
- Department of Anatomy, School of Medicine, Fukuoka University
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- SUZUKI Kazuyoshi
- Department of Endodontics, School of Dentistry, Aichi-Gakuin University
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- HORIBA Naoki
- Department of Endodontics, School of Dentistry, Aichi-Gakuin University
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- NAKAMURA Hiroshi
- Department of Endodontics, School of Dentistry, Aichi-Gakuin University
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- NAKASHIMA Misako
- Center of Advanced Medicine for Dental and Oral Diseases, National Center for Geriatrics and Gerontology Research Institute:Department of Endodontics, School of Dentistry, Aichi-Gakuin University
Bibliographic Information
- Other Title
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- 超音波とナノバブルを用いた薬剤導入による新しい根管内無菌化法の開発
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Abstract
The main goal of root canal treatment is to disinfect the root canal. Proper cleansing of the root canal space is considered essential for success in endodontics. The disinfection of the root canal is performed by mechanical enlargement and chemical irrigants. Then an intracanal medicament is applied in the root canal. However, it is very difficult to completely eliminate bacteria from the root canal system, including those regions where access is difficult, such as canal irregularities, bifurcation, isthmus, fin, and the like. Excess enlargement of the root canal causes root fracture and may necessitate future tooth extraction and has a great influence on a prognosis. Thus, this study was performed to develop a novel method for complete disinfection of the root canal system. Optimal conditions for ultrasound and nanobubbles were examined to deliver medicaments deeper to the dentinal tubules of root canals. Nanobubbles of 0.2-0.3 μm in diameter and with a final concentration of 5% were superior to those of 10 %, and a ultrasound voltage 30 V was superior compared with 31 V to deliver medicaments. The root canals of extracted dog's teeth were infected by GFP-labeled Enterococcus faecalis in the dentinal tubules for 72 hours, and then ultrasound treatment was performed. Disinfection was examined in sections, 150 μm in thickness, after 48 hours-cultivation by confocal laser microscopy. As a result, a significant reduction of GFP fluorescence was seen by application of medicaments into the root canal with ampicillin together with 5% nanobubbles and ultrasound at a voltage of 30 V for 120 seconds compared with medication alone or in conjunction with nanobubbles and ultrasound. These results suggested that medicaments could be delivered deeply in the dental tubules using nanobubbles together with ultrasound leading to disinfection of the intracanal space in a short time.
Journal
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- The Japanese Journal of Conservative Dentistry
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The Japanese Journal of Conservative Dentistry 54 (6), 432-441, 2011
The Japanese Society of Conservative Dentistry
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Details 詳細情報について
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- CRID
- 1390001205520249984
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- NII Article ID
- 110009419172
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- NII Book ID
- AN00191201
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- ISSN
- 21880808
- 03872343
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- Text Lang
- ja
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- Data Source
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- JaLC
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed