Experimental Investigations of Cryopreservation Mechanisms on Dispersed Rat Cardiac Myocytes with Dimethyl Sulfoxide
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- UCHIDA Tsutomu
- Division of Applied Physics, Faculty of Engineering, Hokkaido University
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- MIYAMURA Kenichiro
- Division of Applied Physics, Faculty of Engineering, Hokkaido University
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- NAGAYAMA Masafumi
- Division of Applied Physics, Faculty of Engineering, Hokkaido University
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- GOHARA Kazutoshi
- Division of Applied Physics, Faculty of Engineering, Hokkaido University
Bibliographic Information
- Other Title
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- Dimethyl sulfoxideによる分散ラット心筋細胞の凍結保護機構の実験的検討
- Dimethyl sulfoxide ニ ヨル ブンサン ラット シンキン サイボウ ノ トウケツ ホゴ キコウ ノ ジッケンテキ ケントウ
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Abstract
Dimethyl sulfoxide (DMSO) is one of the useful cryoprotective agents for a variety of cells and tissues allowing prolonged storage at subzero temperatures. Since most of the optimum DMSO concentrations for the different cells and tissues cryopreservations are similar, we predict the reason for the optimum DMSO concentration by measuring the thermodynamic properties of culture medium including DMSO and by observing the toxicity of DMSO on the neonatal rat cardiac myocytes. The melting point measurements suggest that the larger DMSO concentration medium has lower melting points, which would prevent the cell damage by ice reformation during the thawing process. However, such medium has higher toxic effect on the cryopreserved cells. These two contrary factors can predict the optimum concentration of DMSO in the medium for the cryopreservation of cells including the cardiac myocytes.
Journal
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- Cryobiology and Cryotechnology
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Cryobiology and Cryotechnology 58 (1), 59-63, 2012
Japanese Society of Cryobiology and Cryotechnology
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Details 詳細情報について
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- CRID
- 1390001205087652608
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- NII Article ID
- 120005158965
- 110009457809
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- NII Book ID
- AN10448734
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- ISSN
- 24241555
- 13407902
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- HANDLE
- 2115/51700
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- NDL BIB ID
- 023746712
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- Text Lang
- ja
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- Data Source
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- JaLC
- IRDB
- NDL
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed