イオン液体を用いた新規凍結防御材料の可能性 : イオン液体-水混合溶液中のタンパク質の構造安定性

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タイトル別名
  • Possibility of Ionic Liquid for a New Preservation Agent : Structural Stability of Protein in Ionic Liquid-Water Mixed Solutions
  • イオン エキタイ オ モチイタ シンキ トウケツ ボウギョ ザイリョウ ノ カノウセイ : イオン エキタイ-ミズ コンゴウ ヨウエキ チュウ ノ タンパクシツ ノ コウゾウ アンテイセイ

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We have investigated the additive effect of 1-butyl-3-methylimidazolium acetate ([bmim][acetate]), which is one of the imidazolium-based ionic liquid (IL), on the stability of secondary structure of lysozyme in water by the use of FT-IR spectroscopy. From the IR spectral analyses, we found that the population of the α-helical structure of lysozyme decreases up to 6 M. However, the population of the α-helical structure at 10 M is higher than that at 6 M. This result means that the degree of protein unfolding becomes smaller above 6 M. Our result shows that [bmim][acetate] has a possibility for a new preservation agent.

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