乳酸菌配合歯磨剤(アバンビーズ®)の口腔衛生改善効果についての臨床研究  [in Japanese] Clinical Study on the Improvement Effect of a Lactic Acid Bacterium-containing Dentifrice (Avantbise®) on Oral Hygiene  [in Japanese]

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Author(s)

    • 畑野 優子 HATANO Yuko
    • 福岡歯科大学総合歯科学講座総合歯科学分野 Section of General Dentistry, Department of General Dentistry, Fukuoka Dental College
    • 鈴木 奈央 SUZUKI Nao
    • 福岡歯科大学総合歯科学講座総合歯科学分野 Section of General Dentistry, Department of General Dentistry, Fukuoka Dental College
    • 米田 雅裕 YONEDA Masahiro
    • 福岡歯科大学総合歯科学講座総合歯科学分野 Section of General Dentistry, Department of General Dentistry, Fukuoka Dental College
    • 廣藤 卓雄 HIROFUJI Takao
    • 福岡歯科大学総合歯科学講座総合歯科学分野 Section of General Dentistry, Department of General Dentistry, Fukuoka Dental College

Abstract

目的:Enteyococcus faecium WB2000株配合歯磨剤による口腔清掃が口腔内環境に与える影響について,二重盲検式ランダム化比較試験を行いて評価した.方法:本研究に対してインフォームドコンセントが得られた成人のボランティア64名を被験者とした.被験者はE. faecium WB2000株配合歯磨剤(実験群)あるいはE. faecium WB2000株無配合歯磨剤(プラセボ群)を使用して,4週間口腔清掃を行った.実験開始前日(ベースライン),実験開始2,4週後に口腔内診査と唾液採取を行い,口腔内環境の変化を評価した.また採取した唾液より細菌DNAを調製し,Real-time PCR法を用いてStreptococcus mutans, Streptococcus sobrinus, E. faecium, Poyphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Fusobacteyium spp., Prevotella intermedia, Tyeponema denticolaの定量解析を行った.結果:分析対象者59名の内訳は実験群30名(男性25名,女性5名,平均年齢44.3±3.9歳),プラセボ群29名(男性22名,女性7名,平均年齢43.9±4.2歳)であり,ベースラインにおいて年齢,性別,喫煙習慣,口腔内診査項目における群間の違いはみられなかった.実験群では4週後に有意な唾液量の増加と唾液緩衝能の改善がみられた.プラーク付着スコアは実験群では2週後に,プラセボ群では4週後に有意な減少がみられた.歯周病罹患指数はプラセボ群で4週後に有意な増加がみられたが,実験群では変化しなかった.唾液中の総菌数に占める菌種の割合について比較したところ,う蝕原因菌については,実験群でS. sobrinusが有意に減少し,プラセボ群で有意な増加がみられた.歯周病原細菌については,両群でA. actinomycetemcomitansとP. intermediaの減少,実験群でT. denticola,プラセボ群でFusobacterium spp.の増加がみられた.E. faeciumの割合は,両群ともに有意な減少を示した.結論:E. faecium WB2000株配合歯磨剤による口腔清掃は,唾液量の増加,唾液緩衝能の改善,プラーク付着抑制,S. sobyinusの繁殖抑制などの口腔衛生改善効果があり,う蝕予防に役立つ可能性が示唆された.

Objective: The effect of brushing teeth with dentifrice containing Enterococcus faecium WB2000 on oral health was assessed in a double-blind randomized study. Materials and Methods: This study included 68 adult volunteers who provided informed consent to participate in the research project. The subjects cleaned their teeth with a dentifrice containing E. faecium WB2000 or placebo for four weeks. Oral assessment and saliva collection were performed on the day before study (baseline), and after two weeks and four weeks, and changes in oral health were evaluated. Quantitative analyses of the oral bacteria Streptococcus mutans, Streptococcus sobrinus, E. faecium, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Fusobacterium spp., Prevotella intermedia and Treponema denticola in saliva samples were performed by the real-time polymerase chain reaction. Results: A total of 59 subjects including 30 (25 males, 5 females; mean age 44.3 ± 3.9 years) in the experimental group and 29 (22 males, 7 females; mean age 43.9 ± 4.2 years) in the placebo group were analyzed. There were no differences in age, gender, smoking habit, or oral clinical parameters between the two groups. In the experimental group, the amount of saliva significantly increased and salivary buffering capacity significantly improved after four weeks. The degree of plaque adherence was significantly decreased after two weeks in the experimental group and after four weeks in the placebo group. The index for periodontitis was significantly increased after four weeks in the placebo group, but was unchanged in the experimental group. Quantitative analyses of salivary bacteria revealed that the proportion of S. sobrinus, a cariogenic bacterium, was significantly decreased in the experimental group, but significantly increased in the placebo. Concerning periodontopathic bacteria, the proportions of A. actinomycetemcomitans and P intermedia significantly decreased in both groups, those of T denticola increased in the experimental group, and those of Fusobacterium spp. increased in the placebo group. The proportion of E. faecium significantly decreased in both groups. Conclusion: Brushing of teeth using the dentifrice containing E. faecium WB2000 improved oral health, as measured by an increased volume of saliva, significantly improved salivary buffering capacity, decreased dental plaque attachment, and reduced proportion of S. sobrinus, suggesting an anti-cariogenic effect in the oral cavity.

Journal

  • The Japanese Journal of Conservative Dentistry

    The Japanese Journal of Conservative Dentistry 55(3), 219-226, 2012

    The Japanese Society of Conservative Dentistry

References:  25

Codes

  • NII Article ID (NAID)
    110009479999
  • NII NACSIS-CAT ID (NCID)
    AN00191201
  • Text Lang
    JPN
  • Article Type
    ART
  • ISSN
    0387-2343
  • Data Source
    CJP  NII-ELS  J-STAGE 
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