Further analysis on lidocaine-induced increase in intracellular Zn²⁺ concentration : Cytometric model study using rat thymocytes and some fluorescent probes Further analysis on lidocaine-induced increase in intracellular Zn^<2+> concentration : Cytometric model study using rat thymocytes and some fluorescent probes

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Lidocaine augmented FluoZin-3 fluorescence of rat thymocytes, suggesting the increase in intracellular Zn2+concentration. However, the mechanism for lidocaine-induced increase in the intensity of FluoZin-3fluorescence was not elucidated. In this study, in order to reveal the mechanism, the effects of lidocaine onFluoZin-3 and 5-chloromethylfluorescein (5-CMF) fluorescence were examined under various conditions.5-CMF fluorescence was used as a parameter for cellular content of nonprotein thiols. The lidocaine-inducedaugmentation of FluoZin-3 fluorescence was observed under external Ca2+- and/or Zn2+-free conditions,suggesting that lidocaine induced intracellular Zn2+ release. Lidocaine attenuated 5-CMF fluorescence,suggesting the decrease in cellular content of nonprotein thiols. Since some peptides consisting of SH-grouphave been proposed to store Zn2+, the decrease in nonprotein thiol content by

収録刊行物

  • 徳島大学総合科学部自然科学研究

    徳島大学総合科学部自然科学研究 26, 7-10, 2012

    徳島大学

各種コード

  • NII論文ID(NAID)
    110009517148
  • NII書誌ID(NCID)
    AN10065859
  • 本文言語コード
    ENG
  • 資料種別
    Departmental Bulletin Paper
  • 雑誌種別
    大学紀要
  • ISSN
    09146385
  • NDL 記事登録ID
    026939663
  • NDL 請求記号
    Z14-1395
  • データ提供元
    NDL  NII-ELS  IR 
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