麹菌グルタミン酸デカルボキシラーゼ遺伝子の発現様式の検討 [in Japanese] Expression patterns of glutamate decarboxylase genes in Aspergillus oryzae [in Japanese]
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- 新田 陽子 Nitta Yoko
- 兵庫県立大学環境人間学部 School of Human Science and Environment, University of Hyogo
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- 寺畑 吏得子 Terahata Rieko
- 奈良女子大学生活環境学部 Laboratory of Applied Microbiology and Biochemistry, Nara Women's University
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- 松葉 直子 [他] Matsuba Naoko
- 奈良女子大学生活環境学部 Laboratory of Applied Microbiology and Biochemistry, Nara Women's University
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- 永廣 美代子 Nagahiro Miyoko
- 奈良女子大学生活環境学部 Laboratory of Applied Microbiology and Biochemistry, Nara Women's University
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- 牧野 舞 Makino Mai
- 奈良女子大学生活環境学部 Laboratory of Applied Microbiology and Biochemistry, Nara Women's University
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- 吉村 美紀 Yoshimura Miki
- 兵庫県立大学環境人間学部 School of Human Science and Environment, University of Hyogo
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- 佐野 元昭 Sano Motoaki
- 金沢工業大学ゲノム生物工学研究所 Genome Biotechnology Laboratory, Kanazawa Institute of Technology
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- 尾関 健二 Ozeki Kenji
- 金沢工業大学ゲノム生物工学研究所 Genome Biotechnology Laboratory, Kanazawa Institute of Technology
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- 植野 洋志 Ueno Hiroshi
- 奈良女子大学生活環境学部 Laboratory of Applied Microbiology and Biochemistry, Nara Women's University
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Author(s)
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- 新田 陽子 Nitta Yoko
- 兵庫県立大学環境人間学部 School of Human Science and Environment, University of Hyogo
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- 寺畑 吏得子 Terahata Rieko
- 奈良女子大学生活環境学部 Laboratory of Applied Microbiology and Biochemistry, Nara Women's University
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- 松葉 直子 [他] Matsuba Naoko
- 奈良女子大学生活環境学部 Laboratory of Applied Microbiology and Biochemistry, Nara Women's University
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- 永廣 美代子 Nagahiro Miyoko
- 奈良女子大学生活環境学部 Laboratory of Applied Microbiology and Biochemistry, Nara Women's University
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- 牧野 舞 Makino Mai
- 奈良女子大学生活環境学部 Laboratory of Applied Microbiology and Biochemistry, Nara Women's University
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- 吉村 美紀 Yoshimura Miki
- 兵庫県立大学環境人間学部 School of Human Science and Environment, University of Hyogo
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- 佐野 元昭 Sano Motoaki
- 金沢工業大学ゲノム生物工学研究所 Genome Biotechnology Laboratory, Kanazawa Institute of Technology
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- 尾関 健二 Ozeki Kenji
- 金沢工業大学ゲノム生物工学研究所 Genome Biotechnology Laboratory, Kanazawa Institute of Technology
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- 植野 洋志 Ueno Hiroshi
- 奈良女子大学生活環境学部 Laboratory of Applied Microbiology and Biochemistry, Nara Women's University
Abstract
The genome sequencing of Aspergillus oryzae completed in 2005 predicted eight genes of glutamate decarboxylase (GAD) isoforms. A detailed amino acid-sequence analysis of those GAD isoforms and gene expression study were performed. The homology of eight A. oryzae GAD isoforms was compared with those of other species. The isoforms could be divided into four groups based on identities of their amino acid sequences. Two of the isoforms had 31% homology with human GAD 65; two of the isoforms had 55 and 58% homology with rice GAD 1, respectively; two of the isoforms had 45 and 48% homology with yeast (Pichia stipites), respectively; two of the isoforms were unidentified. RT-PCR analysis showed that eight GAD genes were expressed in A. oryzae cultured in the rice solid medium, while four GAD genes were expressed in A. oryzae cultured in the yeast liquid medium. The presence of the eight genes for GAD isoforms in A. oryzae suggests that GADs must be utilized for specific purposes, which should be explored in future studies.
Journal
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- VITAMINS
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VITAMINS 86(9), 508-514, 2012
THE VITAMIN SOCIETY OF JAPAN
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