ナノ銀粒子が細胞に与える影響 : 2次元および3次元培養の比較

• 橋本 正則

  北医療大・歯・生体材料

• 戸島 洋和

  北医療大・歯・生体材料

• 米澤 徹

  北大院・工・材料科学

• 河合 功治

  ミヨシ油脂(株)

• 加我 正行

  北医療大・歯・生体材料

• 遠藤 一彦

  北医療大・歯・生体材料

書誌事項

タイトル別名

• Effect of silver nanoparticles on cells : Comparison between 2 and 3 dimension culture systems

抄録

This study evaluated biological responses of cultured murine macrophages (RAW264.7) to PVP-coated silver nanoparticles (nAg-PVP) using assay of WST-8, LDH release, RT-qPCR, and morphological analyses. Cell culture was conducted using traditional two-dimensional culture (2D) and three-dimensional spheroid culture system (3D). The NPs were taken up by receptor-mediated endocytosis, in which particles aggregated in the vesicles. Cell spheroid formation was obviously observed after 4 and s days culture. The cytotoxic effects of cells in 3D were lower than that in 2D.

収録刊行物

• 日本歯科理工学会誌 = The journal of the Japanese Society for Dental Materials and Devices

  日本歯科理工学会誌 = The journal of the Japanese Society for Dental Materials and Devices 32 (2), 86-, 2013-03-25

  一般社団法人日本歯科理工学会

詳細情報

• CRID: 1570291227972911232
• NII論文ID: 110009596385
• NII書誌ID: AA12452694
• ISSN: 18844421
• Web Site: https://search.jamas.or.jp/link/ui/2014043074
• 本文言語コード: ja
• データソース種別:
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