過酸化尿素の齲蝕原因菌に対する抗菌効果  [in Japanese] Antibacterial Effects of Carbamide Peroxide against Cariogenic Bacteria  [in Japanese]

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Author(s)

    • 鈴木 英明 SUZUKI Hideaki
    • 日本大学松戸歯学部保存修復学講座 Department of Operative Dentistry, Nihon University School of Dentistry at Matsudo
    • 鈴木 義純 SUZUKI Yoshizumi
    • 日本大学松戸歯学部保存修復学講座 Department of Operative Dentistry, Nihon University School of Dentistry at Matsudo
    • 岡田 珠美 OKADA Tamami
    • 日本大学松戸歯学部保存修復学講座 Department of Operative Dentistry, Nihon University School of Dentistry at Matsudo
    • 神谷 直孝 KAMIYA Naotaka
    • 日本大学松戸歯学部保存修復学講座 Department of Operative Dentistry, Nihon University School of Dentistry at Matsudo
    • 森 俊幸 MORI Toshiyuki
    • 日本大学松戸歯学部保存修復学講座 Department of Operative Dentistry, Nihon University School of Dentistry at Matsudo
    • 藤田 光 FUJITA Kou
    • 日本大学松戸歯学部保存修復学講座 Department of Operative Dentistry, Nihon University School of Dentistry at Matsudo
    • 池見 宅司 IKEMI Takuji
    • 日本大学松戸歯学部保存修復学講座 Department of Operative Dentistry, Nihon University School of Dentistry at Matsudo

Abstract

目的:過酸化尿素は,ホワイトニングに使用する薬剤に含まれており,主にホームホワイトニング剤に用いられている薬剤である.過酸化尿素の作用機序は,尿素と過酸化水素に解離し,活性酸素を放出することにより,着色や変色の原因になっている物質に作用し漂白することで歯を白くさせることが知られている.元来,この過酸化水素・過酸化尿素の両薬剤はホワイトニング用としてではなく,口腔用殺菌剤として使用されており,そのうえ,毒性や副作用をもたない安全性の高い薬剤といわれている.ホームホワイトニングで頻用されている過酸化尿素の齲蝕予防の可能性を検討する目的で,その抗菌作用についてin vitroにて実験を行った.材料と方法:実験には,Streptococcus mutans PS-14 (c)株,Streptococcus sobrinus 6715 (d)株,Actinomyces naeslundii ATCC 19246株を用い,10倍段階法にて最小発育阻止濃度の計測を行った.また,Resting cellに対する殺菌作用を濃度的変化ならびに経時的変化について検討した.さらに,不溶性グルカン生成阻害試験としてglucosyltransferase活性値の測定を行った.成績:1.S. mutansに対する最小発育阻止濃度は250μg/mlであった.2.S. sobyinusに対する最小発育阻止濃度は300μg/mlであった.3.A. naeslundiiに対する最小発育阻止濃度は300μg/mlであった.4.過酸化尿素の抗菌作用はS. mutans, S. sobrinusおよびA. naeslundiiのResting cellに対して殺菌的であった.5.過酸化尿素はS. mutans PS-14株ならびにS. sobrinus 6715株産生粗glucosyltransferaseのsucrose依存性不溶性グルカン合成活性を顕著に阻害した.結論:以上のことより過酸化尿素は,齲蝕原因菌に対して顕著な殺菌作用が認められ,抗齲蝕作用を有することが示唆された.

Purpose: Carbamide peroxide is included in medical whitening agents, especially for home-use whitening agents. As its mechanism of action, carbamide peroxide is known to dissociate into urea and hydrogen peroxide. Subsequently, it extracts active oxygen, which affects and bleaches out various substances that discolor or otherwise affect teeth, thereby whitening them. Both hydrogen peroxide and carbamide peroxide, which were originally used as oral bactericides, not for whitening, are considered to be safe agents without toxic consequences or side effects. On the issue of whether urea hydrogen peroxide, which is frequently used for home whitening, can prevent caries or not, we researched its antibacterial action using in vitro experiments. Methods: Using Streptococcus mutans PS-14 strains, Streptococcus sobrinus 6715 strains, and Actinomyces naeslundii ATCC19246 strains, we measured the minimum growth-inhibitory density with the 10-times serial dilution method. Furthermore, we examined their antibacterial action against resting cells in terms of changes in concentration and over time. In addition, we took the measured value of GTF activity as a WIG production inhibition test. Results: 1. The minimum inhibitory concentration against S. mutans was found to be 250 μg/ml. 2. The minimum inhibitory concentration against S. sobrinus was found to be 300 μg/ml. 3. The minimum inhibitory concentration against A. naeslundii was found to be 300 μg/ml. 4. The antibacterial activities were found to be a bactericidal effect against resting cells of S. mutans, S. sobrinus and A. naeslundii. 5. Carbamide peroxide considerably inhibited the sucrose-dependent water insoluble glucan synthesis activity of crude glucosyltransferase produced by S. mutans PS-14 and S. sobrinus 6715. Conclusions: From the above results, carbamide peroxide was found to have a prominent bactericidal effect against cariogenic bacteria, suggesting that it has an anticariogenic effect.

Journal

  • The Japanese Journal of Conservative Dentistry

    The Japanese Journal of Conservative Dentistry 55(6), 373-380, 2012

    The Japanese Society of Conservative Dentistry

References:  44

Codes

  • NII Article ID (NAID)
    110009596769
  • NII NACSIS-CAT ID (NCID)
    AN00191201
  • Text Lang
    JPN
  • Article Type
    ART
  • ISSN
    0387-2343
  • Data Source
    CJP  NII-ELS  J-STAGE 
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