Cytotoxicity of Silane Coupling Agents with Hydrophobic Groups

• OHASHI Katsura

  Division of Restorative Dentistry, Department of Oral Medicine, Kanagawa Dental College

• NIHEI Tomotaro

  Division of Restorative Dentistry, Department of Oral Medicine, Kanagawa Dental College

• MIYAKE Kaori

  Division of Restorative Dentistry, Department of Oral Medicine, Kanagawa Dental College

• SHIMIZU Tota

  Division of Prosthetics, Department of Oral and Maxillofacial Rehabilitation, Kanagawa Dental College

• TERANAKA Ayako

  Division of Restorative Dentistry, Department of Oral Medicine, Kanagawa Dental College

• SERITA Eri

  Division of Restorative Dentistry, Department of Oral Medicine, Kanagawa Dental College

• HARA Kenichiro

  Division of Restorative Dentistry, Department of Oral Medicine, Kanagawa Dental College

• KONDO Yukishige

  Department of Industrial Chemistry, Tokyo University of Science

• YOSHINO Norio

  Department of Industrial Chemistry, Tokyo University of Science

• TERANAKA Toshio

  Division of Restorative Dentistry, Department of Oral Medicine, Kanagawa Dental College

Bibliographic Information

Other Title

• 疎水性基含有シランカップリング剤の細胞毒性

Abstract

Purpose: We developed novel silane coupling agents with hydrophobic groups to improve the water resistance of the coupling layer, and reported that these compounds showed no significant decrease compared with 3-methacryloylxytrimethoxysilane (3-MPS) alone in tensile bond strength after long-term water storage. The aim of this study was to investigate the cytotoxicity of the silane coupling agents nonafluorohexyltrimethoxysilane (4F) and 3- (4-methacryloyloxyphenyl) propyltrimethoxysilane (p-MBS). Methods: Glass plates were modified with 50 mmol/l solutions of 3-MPS, 4F and p-MBS, and then sterilized with ethylene oxide gas. The modified glass plates were immersed in cell culture medium (MO5) for 24 hours at 37℃ in a 5% CO₂ atmosphere to measure the toxicity of the extraction. The extracted medium (100%) was diluted with the MO5 into ten different concentrations (0.5-50%). Chinese Hamster fibroblast (V79) suspension (0.5 ml: 100 cells/ml) was seeded on a 24-well cell culture plate and pre-incubated for 6 hours at 37℃ in a 5% CO₂ atmosphere. After the pre-incubation, 0.5 ml of each concentration of the silane extracts was poured onto the cell culture. After cultivation for 6 days, the cells were stained with 0.1% methylene blue solution and colonies of more than 50 cells were counted. The cytotoxicity of the silane coupling agents was evaluated according to the 50% inhibitory concentration (IC₅₀). Results: The IC₅₀ values of 3-MPS, 4F, and p-MBS were all over 100%. Conclusion: These results suggest that silane coupling agents with hydrophobic groups are not cytotoxic.

Journal

• The Japanese Journal of Conservative Dentistry

  The Japanese Journal of Conservative Dentistry 56 (1), 25-30, 2013

  The Japanese Society of Conservative Dentistry

Keywords

• Cytotoxicity
• Silane coupling agent
• Hydrophobic group

Details

• CRID: 1390001205520722304
• NII Article ID: 110009596778
• NII Book ID: AN00191201
• DOI: 10.11471/shikahozon.56.25
• ISSN: 21880808
• ISSN: 03872343
• Web Site: https://search.jamas.or.jp/link/ui/2013180935
• Text Lang: ja
• Data Source:
  • JaLC
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed