親水性イオン液体Choline Dihydrogen Phosphateを用いたタンパク質のStructural Reversibility

• 竹清 貴浩

  防衛大学校応用化学科

• 山崎 久美子

  防衛大学校応用化学科

• 吉村 幸浩

  防衛大学校応用化学科

書誌事項

タイトル別名

• Structural Reversibility of Protein by the use of a Hydrophilic Ionic Liquid; Chloline Dihydrogen Phosphate
• シン スイセイ イオン エキタイ Choline Dihydrogen Phosphate オ モチイタ タンパクシツ ノ Structural Reversibility

抄録

We have investigated low temperature-induced structural reversibility of ribonuclease A (RNase A) in aqueous choline dihydrogen phoshpahte ([Chol][dhp]) solutions (X(mol%D_2O)=100〜70) by the uses of FTIR and Raman spectroscopies. From the IR spectral analyses, we found that the structural stability of RNase A remains unchanged in the concentration of [Chol][dhp] region from X=97 to 85. Moreover the secondary and tertiary structures of RNase A showed good reversibility upon cooling (77 K) in the same concentration region. On the other hand, Raman OD stretching spectra showed that the aqueous [Chol][dhp]-RNase A solutions take into the glassy state at 77 K in the region from X=90 to 70. On the basis of these results, in the concentration region from X=90 to 85, the aqueous [Chol][dhp]-RNase A solutions have a possibility of good cryoprospectant for aqueous protein solutions.

収録刊行物

• 低温生物工学会誌

  低温生物工学会誌 58 (2), 147-151, 2012

  低温生物工学会

キーワード

• タンパク質
• 構造回復
• イオン液体

詳細情報

• CRID: 1390282680064272768
• NII論文ID: 110009612928
• NII書誌ID: AN10448734
• DOI: 10.20585/cryobolcryotechnol.58.2_147
• ISSN: 24241555
• ISSN: 13407902
• NDL書誌ID: 024143075
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024143075
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • NDL
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可