子嚢菌由来不凍タンパク質の機能解析  [in Japanese] Functional Analysis of Antifreeze Protein from Ascomycete  [in Japanese]

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Author(s)

    • 深見 大地 FUKAMI Daichi
    • 北海道大学大学院生命科学院生命融合科学コース Transdisciplinary Life Science Course, Graduate School of Life Science, Hokkaido University
    • 花田 祐一 HANADA Yuichi
    • 北海道大学大学院生命科学院生命融合科学コース Transdisciplinary Life Science Course, Graduate School of Life Science, Hokkaido University
    • 成 晶 [他] CHENG Jing
    • 北海道大学大学院生命科学院生命融合科学コース Transdisciplinary Life Science Course, Graduate School of Life Science, Hokkaido University
    • 津田 栄 TSUDA Sakae
    • 北海道大学大学院生命科学院生命融合科学コース:産業技術総合研究所生物プロセス研究部門 Transdisciplinary Life Science Course, Graduate School of Life Science, Hokkaido University:Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology(AIST)
    • 近藤 英昌 KONDO Hidemasa
    • 北海道大学大学院生命科学院生命融合科学コース:産業技術総合研究所生物プロセス研究部門 Transdisciplinary Life Science Course, Graduate School of Life Science, Hokkaido University:Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology(AIST)

Abstract

Antifreeze protein (AFP) specifically binds to ice crystal surface and inhibits its growth. In order to elucidate ice-binding mechanism of fungal AFPs, we performed measurements of thermal hysteresis (TH) activity and a recently developed method called "Fluorescence-based Ice Plane Affinity (FIPA)" analysis against AFP identified from Antarctic ascomycete, Antarctomyces psychrotrophicus (AnpAFP). AnpAFP showed a maximum TH of 0.8℃ and led to an ice growth along with the c-axis, which were typical for AFP with moderate antifreeze activity. In addition, FIPA analysis showed that AnpAFP bound to only prism planes of an ice crystal. Such an ice-binding manner of AnpAFP is different from that of the other known microbial AFPs, since they can bind to both prism and basal planes.

Journal

  • Cryobiology and Cryotechnology

    Cryobiology and Cryotechnology 59(2), 157-160, 2013

    Japanese Society of Cryobiology and Cryotechnology

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