根管治療に用いられる仮封材の口腔内細菌に対する抗菌性  [in Japanese] Antibacterial Activity of Endodontic Restorative Materials against Oral Bacteria  [in Japanese]

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Abstract

目的:歯科治療に頻用されている歯科用セメント,覆髄材ならびに仮封材には,口腔内常在菌の窩洞内への侵入を防止し,窩洞内を無菌的に保つために抗菌性を保有することが望ましいといわれている.本研究では,臨床で頻繁に使用される仮封材の口腔内細菌に対する抗菌性について検討を行った.材料と方法:仮封材には,酸化亜鉛ユージノール製剤(ネオダイン-α,ネオ製薬工業),水硬性仮封材(キャビトンEX,ジーシー),タンニン・フッ化物合剤配合水硬性仮封材(ハイ-シール,松風),高分子系仮封材(プラストシールノーマル,日本歯科薬品),光重合型レジン系仮封材(エバダインプラス,ネオ製薬工業)の計5種類を用い,これらを被験材料とした.供試菌株として,Porphyromonas gingivalis ATCC 33277, Fusobacterium nucleatum TDC 100, Parvimonas micra JCM 12970, Streptococcus mutans Ingbritt, Streptococcus sanguinis ATCC 10580を使用した.BHI寒天培地上の被験試料周囲に発現する阻止円の半径(cm)を計測し,各仮封材の被験菌種に対する抗菌性を試料間で比較検討した.統計解析はKruskal-Wallis testを用い,5%の有意水準で解析を行った.成績:ネオダイン-αはすべての菌種において抗菌性が認められた.キャビトンEXはF. nucleatumのみに抗菌性が認められた.ハイ-シールはP. micra以外のP. gingivalis, F. nucleatum, S. mutans, S. sanguinisに抗菌性が認められた.プラストシールはP. gingivalisのみ抗菌性が認められた.エバダインはいずれの菌種に対しても抗菌性が認められなかった.結論:本研究では,各種仮封材における抗菌性に差異が認められたが,仮封材成分の抗菌性によるものであることが示唆された.

Purpose: Elimination of microorganisms from the root canal is necessary to resolve apical periodontitis. Antibacterial activity is required in the temporary filling to protect against entry of bacteria into the root canal. The present study was designed to investigate the antibacterial activity of temporary filling materials against oral bacteria. Methods: Zinc oxide eugenol cement, hydraulic cement, fluorine-containing hydraulic cement, polymeric filling material and photopolymerized material were used. The antibacterial activity of these materials against Porphyromonas gingivalis ATCC 33277, Fusobacterium nucleatum TDC 100, Parvimonas micra JCM 12970, Streptococcus mutans Ingbritt and Streptococcus sanguinis ATCC 10580 were investigated by the diffusion method. Data were analyzed using the Kruskal-Wallis test at a significance level of 5 %. Results: The antibacterial activity against P gingivalis of zinc oxide eugenol cement was significantly higher than those of hydraulic cement and photopolymerized material. Antibacterial activity against F nucleatum was also observed for zinc oxide eugenol cement, hydraulic cement, and fluorine-containing hydraulic cement. Against P micra, only zinc oxide eugenol cement showed antibacterial activity, and against S. mutans and S. sanguinis, antibacterial activity was observed for zinc oxide eugenol cement and fluorine-containing hydraulic cement. High antibacterial activities were observed for zinc oxide eugenol cement and fluorine-containing hydraulic cement, but none of the photopolymerized material specimens showed activity. Conclusion: Antibacterial activity differed between temporary filling materials, suggesting that the antimicrobial constituents of temporary filling materials influence antibacterial activity.

Journal

  • The Japanese Journal of Conservative Dentistry

    The Japanese Journal of Conservative Dentistry 56(2), 138-143, 2013

    The Japanese Society of Conservative Dentistry

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