10 細胞膜損傷および核酸量を指標とした超音波と放射線の殺菌効果の比較(一般講演)

DOI

書誌事項

タイトル別名
  • 10 A comparison between ultrasound and gamma-ray effect on the inactivation of microorganisms considering cell membrane permeability and nucleic acid(Oral presentation)

抄録

The effect of ultrasonic irradiation and gamma-ray radiation on the inactivation of microorganisms is strongly associated with free radicals which generated from water molecule. In this aspect, it is considered that the both effects are seemed to be similar in terms of the radical contribution, especially at a range around 200 kHz for sonication condition. However, in case of ultrasound, the shock wave also contributes for microbial disruption of cell components including cell membrane or genic compound, which is lead to cell death. In this study, we compared the effect of ultrasound and gamma-ray considering the indication of cell membrane permeability and nucleic acid. After each sonication and gamma radiation, the amount of nucleic acid was measured by using BACTANA[○!R] (an automatic microbial analyzer based on flow cytometry) in addition to colony counting. It is observed that the nucleic acid synthesis significantly increased within the cells before entering the logarithmic phase of their growth. Moreover, it was found that the pattern of increasing amount of nucleic acid following with the growth of the cells was different between sonication and gamma-ray radiation. In case of sonication, increasing rate hardly change depending on the sonication time. On the other hand, it was decreased with increasing intensity of gamma-ray radiation. It seems that the damage on survival cell remain after gamma-ray radiation and it takes long time to repair and growth of the cell.

収録刊行物

詳細情報 詳細情報について

  • CRID
    1390282681028156800
  • NII論文ID
    110009710259
  • DOI
    10.20577/pamjss.13.0_62
  • ISSN
    24241512
  • 本文言語コード
    ja
  • データソース種別
    • JaLC
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

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