P33 超音波照射による超音波機能性分子とDNAの結合

DOI

書誌事項

タイトル別名
  • P33 Ultrasonic Binding of the Sonofunctional Molecule to DNA

抄録

We have proposed a concept of 'Sonofunctional Molecules', which develop new functionalities by ultrasonic irradiation. As a first sonofunctional molecule, compound 1 was prepared by introducing alkyl thiol moieties into a diaminoacridine moiety which was known as a DNA intercalator. Compound 1 has the potential for cancer treatment. After intercalation into DNA and successive ultrasonic irradiation, compound 1 can be expected to form irreversible complexes with DNA, which may cause damages on DNA duplication. In the preliminary experiments, after ultrasonic irradiation for several hours in an aqueous i-PrOH, it was confirmed that 1 was oxidized and converted into cyclic structure by the formation of intramolecular disulfide bond. In this study, the binding ability of compound 1 to DNA was examined by means of UV spectroscopy. The typical experimental procedure is as follows. DNA (from Salmon Testes, Sigma) and 1 were dissolved into water-i-PrOH-CH_2Cl_2, and irradiated by 200 kHz ultrasound (Kaijo Model 4021) for 24 hours at 25℃(Fig.1). After the reaction solution was washed with CH_2Cl_2 and its pH was adjusted to 10.0 ±0.5, UV spectra of water layer were measured. As shown in Fig.2, compound 1 was solubilized into the water layer, although 1 had no solubility into water. Moreover, the solubility was doubly enhanced by ultrasonic irradiation. On the other hand, derivatives 2 and 3 showed no ultrasonic enhancement in solubility into water (Figures 3 and 4). These results clearly indicate that the sonofunctional molecule 1 is bound to DNA by ultrasonic irradiation and terminal thiol groups take an important function in the binding.

収録刊行物

詳細情報 詳細情報について

  • CRID
    1390282681029388160
  • NII論文ID
    110009710342
  • DOI
    10.20577/pamjss.14.0_164
  • ISSN
    24241512
  • 本文言語コード
    ja
  • データソース種別
    • JaLC
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

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