P04 二酸化チタン/超音波触媒法による大腸菌膜過酸化脂質の生成と殺菌メカニズム(第II部,ポスター発表)

DOI

書誌事項

タイトル別名
  • P04 Ultrasonically induced membrane lipid peroxidation of Escherichia coli in the presence of TiO_2, towards an understanding of its killing mechanisms(Part II,Poster session)

抄録

Strong bacteriocidal activity of titanium dioxide (TiO_2) irradiated with ultrasound (U.S.) is an established phenomenon. A new non-woven TiO_2 fabric under U.S. system was used to well characterize the sonocatalytic disinfection and lipid peroxidation of Escherichia coli (E. coli) cells membrane and to investigate the possible killing mechanisms of its cell. It was found that applying ultrasonic irradiation with non-woven TiO_2 fabrics reduces the E. coli cell concentration very rapidly, which indicated very high disinfection rate at this system. Diphenyl-1-pyrenylphosphin (DPPP) was used as a non-fluorescent probe for lipid peroxidation on E. coli cells membrane. Reaction of DPPP with U.S. treated samples and organic hydroperoxides were examined by monitoring increase in fluorescence intensity of DPPP oxide (DPPP=O). The mechanism of cell killing and lipid peroxidation were further investigated by examining the effect of OH radical scavenger. The scavenger studies indicated the functional role of hydroxyl (OH) radicals in the initiation of ultrasound induced lipid peroxidation.

収録刊行物

詳細情報 詳細情報について

  • CRID
    1390282681029672960
  • NII論文ID
    110009710405
  • DOI
    10.20577/pamjss.17.0_48
  • ISSN
    24241512
  • 本文言語コード
    en
  • データソース種別
    • JaLC
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

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