P28 TiO_2ナノ粒子と超音波触媒法を併用したがん細胞傷害効果の評価(第II部,ポスター発表)

DOI

書誌事項

タイトル別名
  • P28 Evaluation of sonocatalytic cytotoxicity of cancer cells with incorporation of TiO_2 nanoparticles(Part II,Poster session)

抄録

Our previous study demonstrated that the titanium dioxide (TiO_2) could catalyze production of hydroxyl (OH) radicals by ultrasound (U.S.) irradiation even without any photo-excitation. The purpose of this study was to extend the applicability of our "TiO_2/U.S. system" to drug delivery systems for cancer therapy by modifying the surface of TiO_2 nanoparticles with PreS1/S2 protein for targeting HepG2 cells. To evaluate the specific binding of the preS1/S2-conjugated TiO_2 particles (preS1/S2-TiO_2), liver and colon cancer cell lines, HepG2 and WiDr were incubated with the preS1/S2-TiO_2 (0.01% (v/v)) for 6 hours and subjected to fluorescent immunostaining with anti-preS1 antibody. The fluorescence was detected only in the case of HepG2 cells, indicating specific binding and incorporation of preS1/S2-TiO_2. To evaluate the sonocatalytic cytotoxicity of HepG2 cells with incorporation of preS1/S2-TiO_2, the culture dish was irradiated with U.S. at the frequency of 1MHz and with output power of 0.3 W/m^2 for 30 sec (50% duty). Compared to the control condition without preS1/S2-TiO_2 incorporation and/or U.S. irradiation, the growth of HepG2 cells was restrained with both the preS1/S2-TiO_2 incorporation and the U.S. irradiation at initial time of culture, suggesting our "TiO_2/U.S. system" could be promising tool for cancer therapy.

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詳細情報 詳細情報について

  • CRID
    1390001206052278272
  • NII論文ID
    110009710429
  • DOI
    10.20577/pamjss.17.0_96
  • ISSN
    24241512
  • 本文言語コード
    ja
  • データソース種別
    • JaLC
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

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