S28. Targeted sonodynamic therapy using protein-modified TiO_2 nanoparticles(Oral Presentation)

• Ninomiya Kazuaki

  Institute of Nature and Environmental Technology, Kanazawa University

• Oshima Shuhei

  Division of Material Engineering, Graduate School of Natural Science and Technology, Kanazawa University

• Sonoke Shiro

  Division of Material Engineering, Graduate School of Natural Science and Technology, Kanazawa University

• Ogino Chiaki

  Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University

• Kuroda Shun-ichi

  Department of Bioengineering Sciences, Graduate School of Bioagricultural Sciences, Nagoya University

• Shimizu Nobuaki

  Institute of Nature and Environmental Technology, Kanazawa University

抄録

Our previous study suggested new sonodynamic therapy for cancer cells based on the delivery of titanium dioxide (TiO_2) nanoparticles (NPs) modified with a protein specifically recognizing target cells and subsequent generation of hydroxyl radicals from TiO_2 NPs activated by external ultrasound irradiation (called TiO_2/US treatment). The present study first examined the uptake behavior of TiO_2 NPs modified with pre-S1/S2 (model protein-recognizing hepatocytes) by HepG2 cells for 24 h. Next, the effect of the TiO_2/US treatment on HepG2 cell growth was examined for 96 h after the 1 MHz ultrasound was irradiated (0.1 W/cm^2, 30 s) to the cells which incorporated the TiO_2 NPs. Although no apparent cell-injury was observed until 24 h after the treatment, the viable cell concentration had deteriorated to 46% of the control at 96 h. Finally, the TiO_2/US treatment was applied to a mouse xenograft model. The pre-S1/S2-immobilized TiO_2 (0.1 mg) was directly injected into tumors, followed by 1 MHz ultrasound irradiation at 1.0 W/cm^2 for 60 s. As a result of the treatment repeated 5 times within 13 days, tumor growth could be hampered up to 28 days compared with the control conditions.

収録刊行物

• ソノケミストリー討論会講演論文集

  ソノケミストリー討論会講演論文集 20 (0), 80-83, 2011

  日本ソノケミストリー学会

キーワード

• Ultrasound
• Titanium dioxide
• Nanoparticles
• Pre-S1
• S2
• Sonodynamic therapy

詳細情報

• CRID: 1390001206052548864
• NII論文ID: 110009710550
• DOI: 10.20577/pamjss.20.0_80
• ISSN: 24241512
• 本文言語コード: en
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可