書誌事項
- タイトル別名
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- S021021 Development of a Decellularized Bovine Tendon for Anterior Cruciate Ligament Reconstruction
抄録
Autologous tendons or bone-patellar tendon-bone grafts are clinically used for reconstruction of anterior cruciate ligaments, because of unsatisfactory durability of synthetic materials. The aims of this study are to realize decellularization of 16-cm long xenogeneic tendons which have a potential to guide regeneration, and to investigate influences of decellularization on tissue mechanical properties. Bovine tendons were decellularized by combination of pulsatile flow-and-pressure circulation of 1 wt% deoxycholic acid and microwave radiation. Then, the tissues were additionally treated with endonuclease. Influences of decellularization on durability was investigated by a cyclic tensile test under 0-100 N loads. When the tissues were not ruptured, ultimate strength, elastic modulus, and ultimate strain were examined by a uniaxial tensile test. DNA of 16-cm long bovine tendons was successfully removed over 99.99% by our decellularization technology. Non-treated and decellularized tendons were not ruptured in 1000 cycles' tensile durability tests. After the cyclic tests, ultimate stress, elastic modulus, and ultimate strain of the decellularized tendons were comparable to those of non-treated tendons. We here reported, to our knowledge, the first successful decellularization of 16-cm long tendons with preserving mechanical integrity.
収録刊行物
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- 年次大会
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年次大会 2011 (0), _S021021-1-_S021021-3, 2011
一般社団法人 日本機械学会
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キーワード
詳細情報 詳細情報について
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- CRID
- 1390001205840076160
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- NII論文ID
- 110009759794
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- ISSN
- 24242667
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可