Hybrid sponge comprised of galactosylated chitosan and hyaluronic acid mediates the co-culture of hepatocytes and endothelial cells

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Author(s)

    • Shang Yi Shang Yi
    • Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology
    • Tamai Miho Tamai Miho
    • Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology
    • Nagaoka Noriyuki
    • Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
    • Yoshida Yasuhiro
    • Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
    • Yang Jun
    • The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University
    • Tagawa Yoh-ichi
    • Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology

Abstract

When constructing an in vitro model of liver tissue to mimic the in vivo liver microenvironment, the major challenge is to preserve and maintain the hepatocyte phenotype. The aim of this study was to develop a novel intelligent hybrid sponge for use in a dense co-culture system designed to simulate the liver microenvironment. We prepared a galactosylated chitosan (GCs)/hyaluronic acid (HA) hybrid sponge using a freeze-drying technique for the co-culture of primary hepatocytes and endothelial cells. Subsequently, we investigated the biocompatibility of the GCs/HA scaffold with primary hepatocytes and endothelial cells in terms of cell attachment, morphology, bioactivity, and maintenance of specific liver functions. The GCs/HA-hybrid sponge demonstrated good biocompatibility not only with primary hepatocytes, but also with endothelial cells. In our model, primary hepatocytes exhibited superior bioactivity and higher levels of liver-specific functions in terms of hepatocyte-specific gene expression, urea production, and testosterone metabolism as compared to a monoculture system. We succeeded in constructing a liver tissue-like model using the GCs/HA-hybrid sponge. Therefore, we anticipate that GCs/HA-hybrid sponges may be a promising matrix for the co-culture of hepatocytes and endothelial cells in liver tissue engineering, and might be employed as a novel co-culture model for applications in toxicology and drug metabolism.

Journal

  • Journal of bioscience and bioengineering

    Journal of bioscience and bioengineering 117(1), 99-106, 2014-01

    The Society for Biotechnology, Japan

Codes

  • NII Article ID (NAID)
    110009766612
  • NII NACSIS-CAT ID (NCID)
    AA11307678
  • Text Lang
    ENG
  • ISSN
    1389-1723
  • NDL Article ID
    025159584
  • NDL Call No.
    Z53-S65
  • Data Source
    NDL  NII-ELS  Crossref 
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