Study on of Genetic Diversity of Saccharina japonica for Conservation of Kelp Bed

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  • Saccharina japonicaの遺伝的多様性の保全に関する研究
  • Saccharina japonica ノ イデンテキ タヨウセイ ノ ホゼン ニ カンスル ケンキュウ

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Abstract

In order to conserve kelp bed of Saccharina japonica with maintenance of genetic diversity, we investigated the genetic diversity using AFLP and microsatellite markers, and also searched proteins of which the expression level change depending on environmental condition with the objective of developing diagnostic technic to detect an environmental stress. As results of AFLP analysis, genetic difference was detected between collecting sites. It is recognized that, except for several populations, genetic similarity coefficient (S) between populations was correlated with regional continuity. Developed 10 microsatellite markers could detect polymorphisms between individuals. The results from proteomic profiling, the expression of 51 spots was enhanced under high temperature condition, and of nine spots under oligotrophic condition. The genetic parameters obtained from AFLP analysis suggested that several local populations existed. In other words, extinction of these populations indicated the loss of genetic diversity. Therefore, persistence of them becomes major issue for conservation of genetic diversity of this species. It seems that the diagnostic technic to detect the environmental stress plays a key role in maintaining kelp beds in the future.

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