Effect of coupling factor 6 on chemokine receptors in vascular endothelial cells

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  • Suzuki Akiko
    Department of Cardiology, Respiratory Medicine and Nephrology, Hirosaki University Graduate School of Medicine
  • Osanai Tomohiro
    Department of Cardiology, Respiratory Medicine and Nephrology, Hirosaki University Graduate School of Medicine Department of Hypertension and Stroke Medicine, Hirosaki University Graduate School of Medicine
  • Tanaka Makoto
    Department of Hypertension and Stroke Medicine, Hirosaki University Graduate School of Medicine
  • Endo Tomohide
    Department of Cardiology, Respiratory Medicine and Nephrology, Hirosaki University Graduate School of Medicine
  • Murakami Kazuo
    Department of Cardiology, Respiratory Medicine and Nephrology, Hirosaki University Graduate School of Medicine
  • Tomita Hirofumi
    Department of Cardiology, Respiratory Medicine and Nephrology, Hirosaki University Graduate School of Medicine
  • Okumura Ken
    Department of Cardiology, Respiratory Medicine and Nephrology, Hirosaki University Graduate School of Medicine Department of Hypertension and Stroke Medicine, Hirosaki University Graduate School of Medicine

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Other Title
  • Coupling factor 6 の血管内皮細胞におけるケモカイン受容体発現に及ぼす影響

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Abstract

Objectives: Vascular endothelial cells are exposed to an acidic pH, but its influence on chemokine receptors expressed in the vascular endothelial cells is unclear. We investigated the role of coupling factor 6 (CF6), a novel stimulator of proton importer, in the regulation of chemokine receptors in the vascular endothelial cells.<br> Methods and Results: In microarray analysis, there were the increased expression of CC chemokine receptor 9 (CCR9) and CX3C chemokine receptor 1 (CX3CR1) and the decreased expression of CXC chemokine receptor 4 (CXCR4) in the human umbilical vascular endothelial cells (HUVEC) that were exposed to CF6. The ratio of CXCR4 to GAPDH mRNA was decreased in HUVEC that were exposed to either CF6 at 10⁻⁷M or hypoxia to a similar degree. Apoptotic cells, measured by annexin-V propidium iodide kit, were increased in HUVEC that were exposed to CF6 for 24 hours in normoxia.<br> Conclusions: CF6 influences the expression of chemokine receptors and induces apoptosis in the vascular endothelial cells.

Journal

  • Hirosaki Medical Journal

    Hirosaki Medical Journal 65 (2-4), 119-127, 2014

    Hirosaki University Graduate School of Medicine,Hirosaki Medical Society

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