Osteogenesis related gene expression on titanium surfaces with nano-network structures formed by treatment with alkali solutions

DOI
  • Komasa Satoshi
    Department of Removable Prosthodontics and Occlusion, Osaka Dental University
  • Xing Helin
    Graduate School of Dentistry (Department of Removable Prosthodontics and Occlusion), Osaka Dental University
  • Taguchi Yoichiro
    Department of Periodontology, Osaka Dental University
  • Kayama Tomoki
    Graduate School of Dentistry (Department of Removable Prosthodontics and Occlusion), Osaka Dental University
  • Fujio Miho
    Graduate School of Dentistry (Department of Removable Prosthodontics and Occlusion), Osaka Dental University
  • Miyake Akiko
    Graduate School of Dentistry (Department of Removable Prosthodontics and Occlusion), Osaka Dental University
  • Shimamura Seiji
    Department of Removable Prosthodontics and Occlusion, Osaka Dental University
  • Kusumoto Tetsuji
    Department of Fixed Prosthodontics and Occlusion, Osaka Dental University
  • Nishizaki Hiroshi
    Department of Removable Prosthodontics and Occlusion, Osaka Dental University
  • Okazaki Joji
    Department of Removable Prosthodontics and Occlusion, Osaka Dental University

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We evaluated the ability of titanium nanosheet-modified surfaces of titanium plates to affect cellular gene expression related to osteogenic differentiation. To create the nanostructures, titanium discs were incubated in 10 M NaOH solution at 30℃ for 24 h, and then washed and dried. Cell culture experiments were performed with Sprague-Dawley rat bone marrow cells, which were seeded into microplate wells and cultured in medium designed to induce osteogenic differentiation. We measured alkaline phosphatase (ALP), osterix (OSX), bone morphogenetic protein (BMP), and osteopontin (OPN) gene expression to assess the levels of bone differentiation. After 3 days, cellular ALP and OSX expression was significantly higher in the test group than in the controls. After 7 days, the cells in the test group showed significantly higher BMP and OPN gene expression than those in the controls. These data suggest that titanium implants modified by application of nanostructures promote osteogenic differentiation, and may improve the biointegration of these implants into alveolar bone.

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