Production of plant cell wall-degrading enzymes in Lentinula edodes and the important role of laccase in early stages of solid-state cultivation

DOI IR Web Site 1 Citations
  • NAGAI Masaru
    Iwate Biotechnology Research Center:(Present office)Institute for Environmental Sciences
  • SATO Toshitsugu
    Iwate Biotechnology Research Center:(Present office)Department of Biotechnology and Environmental Chemistry, National University Corporation Kitami Institute of Technology

Bibliographic Information

Other Title
  • シイタケ菌床栽培初期における植物細胞壁分解酵素活性の変動と菌床中ラッカーゼの役割

Search this article

Abstract

We studied the production of plant cell wall-degrading enzymes in Lentinula edodes grown on sawdust medium. A mycelial mat was spotted onto the center of a medium plate, which was then incubated at 23℃ for 31 days. Laccase (EC 1.10.3.2) activity was detected before that of other enzymes. Laccase activity increased along with mycelial growth until the leading edge of the mycelium reached the edge of the plate, and then decreased. Decrease of laccase activity was followed by increased activity of other enzymes. Sawdust treated with purified laccase (Lcc1) was more sensitive to degradation by crude enzymes from L. edodes. These results suggest that laccase plays an important role during early stages of solid-state cultivation of L. edodes.

Journal

Citations (1)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top