Protective Effect of Group3 LEA Model Peptides on Desiccation-induced Liposome Fusion

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  • Furuki Takao
    Center for Biological Resources and Informatics, Tokyo Institute of Technology
  • Sakurai Minoru
    Center for Biological Resources and Informatics, Tokyo Institute of Technology

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  • グループ3LEAタンパク質のモデルペプチドによるリポソームの乾燥融合防止効果

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Abstract

We investigated whether a model peptide for group 3 late embryogenesis abundant (G3LEA) proteins that we had developed previously is capable of protecting liposomes against their fusion under desiccation stress. Four different peptides were tested for comparison: 1) PvLEA-22, which consists of two tandem repeats of the 11-mer motif characteristic of LEA proteins from an African sleeping chironomid; 2) a peptide with amino acid composition identical to that of PvLEA-22, but with its sequence scrambled; 3) poly-L-glutamic acid; and 4) poly-L-lysine. Based upon the results of the particle size distribution measurement of liposomes and the leakage test of a fluorescent probe inside liposomes, we argue that the synthetic 22-mer peptide that derived from its parent G3LEA protein sequence is superior to simple homopeptides as the anti-fusion reagent of liposomes and can achieve the ability of protecting liposome that is comparable to or higher than several native LEA proteins reported previously.

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