2A2-S04 単一細胞解析のための環境制御型電顕内ナノマニピュレータの位置決め自動制御(バイオマニピュレーション)

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  • 2A2-S04 The Position Control of Nano Manipulator for Single Cell Analysis inside an Environmental Scanning Electron Microscope(Bio-Manipulation)

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A nano manipulator position control system was proposed for single cell analysis inside an environmental scanning electron microscopy (ESEM). The nano manipulator inside ESEM has three degrees of freedom, i.e. X, Y and Z translation. The endeffector for single cell analysis was fixed to the nano manipulator, and the single yeast cell was put on a stage substrate inside ESEM. The real time images during the experiment were observed by ESEM system in real time. A median filter was used to remove the image noise. The objects were identified by template matching technique and the positions were calculated by image processing. The position information was used as the feedback signal to control the movement of the nanorobotic manipulator.

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