Role of Zn²⁺ in restoration of nonprotein thiol content in the cells under chemical stress induced by triclocarban Role of Zn^<2+> in restoration of nonprotein thiol content in the cells under chemical stress induced by triclocarban

この論文にアクセスする

この論文をさがす

著者

    • Fukunaga Eri Fukunaga Eri
    • Division of Environmental Symbiosis Studies, Faculty of Integrated Arts and Sciences, The University of Tokushima
    • Oyama Yasuo
    • Division of Environmental Symbiosis Studies, Faculty of Integrated Arts and Sciences, The University of Tokushima

抄録

We have proposed that intracellular Zn2+ release during oxidative stress is a trigger to restore cellular thiol content that is decreased by oxidative stress. Recently, we found that the incubation with triclocarban for 1 h decreased cellular thiol content and increased intracellular Zn2+ concentration. It was reminiscent of the possibility that the increase in intracellular Zn2+ concentration by triclocarban could become a trigger to restore the cellular content of nonprotein thiols. To test the possibility, we cytometrically examined the effects of prolonged incubation (3 h) with triclocarban on the cellular content of nonprotein thiols, presumably glutathione, by the use of 5-chloromethylfluorescein (5-CMF) diacetate, a fluorescent indicator of cellular nonprotein thiols, in rat thymocytes. The intensity of 5-CMF fluorescence after the 3 h incubation with 300 nM triclocarban was significantly higher than that after the 1 h incubation. In the presence of a chelator of intracellular Zn2+, such a significant difference was not observed. The results suggest that the increase in intracellular Zn2+ concentration by triclocarban is one of triggers to restore cellular content that is decreased by triclocarban.

収録刊行物

  • 徳島大学総合科学部自然科学研究

    徳島大学総合科学部自然科学研究 27, 1-5, 2013

    徳島大学

各種コード

  • NII論文ID(NAID)
    110009915516
  • NII書誌ID(NCID)
    AN10065859
  • 本文言語コード
    ENG
  • 資料種別
    Departmental Bulletin Paper
  • 雑誌種別
    大学紀要
  • ISSN
    09146385
  • NDL 記事登録ID
    026939136
  • NDL 請求記号
    Z14-1395
  • データ提供元
    NDL  NII-ELS  IR 
ページトップへ