Nonivamide, a natural analog of capsaicin, affects intracellular Ca²⁺ level in rat thymic lymphocytes Nonivamide, a natural analog of capsaicin, affects intracellular Ca^<2+> level in rat thymic lymphocytes

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著者

    • Fukunaga Eri Fukunaga Eri
    • Laboratory of Cellular Signaling, Graduate School of Integrated Arts and Sciences, The University of Tokushima
    • Kanemaru Kaori
    • Laboratory of Cellular Signaling, Graduate School of Integrated Arts and Sciences, The University of Tokushima
    • Satoh Masaya
    • Laboratory of Cellular Signaling, Graduate School of Integrated Arts and Sciences, The University of Tokushima
    • Oyama Yasuo
    • Laboratory of Cellular Signaling, Graduate School of Integrated Arts and Sciences, The University of Tokushima

抄録

Effect of nonivamide, a natural analog of capsaicin, on intracellular Ca2+ level of rat thymocytes was examined using a flow-cytometric technique with appropriate fluorescent probes in order to further characterize the cytotoxicity because nonivamide can be used as an active intergradient of antifouling paints. Nonivamide at concentrations ranging from 30 μM to 300 μM significantly increased the intensity of Fluo-3 fluorescence. The potency of 100 μM nonivamide to increase the fluorescence was similar to that of 100 μM capsaicin. The increase in Fluo-3 fluorescence by 100 μM nonivamide was attenuated under an external Ca2+-free condition. Nonivamide at 100 μM also increased the intensity of Fluo-3 fluorescence in the continued presence of 100 μM capsaicin. It is suggested that nonivamide at high micromolar concentrations increases intracellular Ca2+ level via the activation of vanilloid receptors. Nonivamide concentrations (30 μM or more) that increase intracellular Ca2+ level in rat thymocytes are comparable to those in algal cells. However, it is something hard to argue the implications in environmental science because nonivamide doesn't seem to be released into environment in such a high concentration, and because bioaccumulation of nonivamide has not been reported.

収録刊行物

  • 徳島大学総合科学部自然科学研究

    徳島大学総合科学部自然科学研究 28, 15-19, 2014

    徳島大学

各種コード

  • NII論文ID(NAID)
    110009915549
  • NII書誌ID(NCID)
    AN10065859
  • 本文言語コード
    ENG
  • 資料種別
    Departmental Bulletin Paper
  • 雑誌種別
    大学紀要
  • ISSN
    09146385
  • NDL 記事登録ID
    026939747
  • NDL 請求記号
    Z14-1395
  • データ提供元
    NDL  NII-ELS  IR 
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