モンゴル国家畜乳および乳製品中の細菌のクローンライブラリー法による解析 Phytogenetic analysis of bacteria from Mongolian animal milks and their dairy products by clone library method

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著者

    • 麻植 優人 Oe Yuto
    • 徳島大学総合科学部社会創生学科 Laboratory of Biochemistry, Faculty of Integrated Arts and Sciences, The University of Tokushima
    • 阿部 桃子 Abe Momoko
    • 徳島大学総合科学部社会創生学科 Laboratory of Biochemistry, Faculty of Integrated Arts and Sciences, The University of Tokushima
    • 遠藤 陽介 [他] Endoh Yousuke
    • 徳島大学大学院総合科学教育部地域科学専攻 Laboratory of Biochemistry,Graduate school of Integrated Arts and Sciences, The University of Tokushima
    • 坂田 真宏 Sakata Masahiro
    • 徳島大学大学院総合科学教育部地域科学専攻 Laboratory of Biochemistry,Graduate school of Integrated Arts and Sciences, The University of Tokushima
    • 大橋 眞 Ohashi Makoto
    • 徳島大学大学院ソシオ・アーツ・アンド・サイエンス研究部 Institute of Socio-Arts and Sciences, The University of Tokushima
    • 佐藤 高則 Satoh Takanori
    • 徳島大学大学院ソシオ・アーツ・アンド・サイエンス研究部 Institute of Socio-Arts and Sciences, The University of Tokushima

抄録

Various animal milks and their dairy products like AIRAG (fermented horse milk) and yogurt are very popular among Mongolian people. The climate of Mongolia is very severe, and they preserve their health by the intake of above animal milks and dairy products. In this study, we explored the diversity of bacteria in Mongolian animal milks (Cow, Horse, Goat, and Camel), AIRAG, and Camel milk yogurt by the clone library method of their 16S rRNA genes.Firstly, we prepared the whole genomic DNA from four animal milks and two dairy products, and amplified each 16S rRNA genes by PCR. PCR products (about 1.4kbp) were cloned into pGEM-T vector, and analyzed DNA sequences of totally 79 clones from AIRAG, Camel milk yogurt, and four animal milks. It was revealed that homologous clones to Lactobacillus helveticus are dominant in the clone libraries of both dairy products, whereas the other clones to Lactococcus from AIRAG, and Acetobacter from camel milk yogurt. Furthermore, We found that homologous clones to Lactococcus are dominant in Mongolian cow and horse milks, whereas Leuconostoc in camel milk. Therefore, it might be useful information for screening the bioactive strains from milk products in Mongolia.

収録刊行物

  • 徳島大学総合科学部自然科学研究

    徳島大学総合科学部自然科学研究 28, 27-36, 2014

    徳島大学

各種コード

  • NII論文ID(NAID)
    110009915554
  • NII書誌ID(NCID)
    AN10065859
  • 本文言語コード
    JPN
  • 資料種別
    Departmental Bulletin Paper
  • 雑誌種別
    大学紀要
  • ISSN
    09146385
  • NDL 記事登録ID
    026939788
  • NDL 請求記号
    Z14-1395
  • データ提供元
    NDL  NII-ELS  IR 
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