OS0610 細胞-ECM間の相互作用の解明に向けた3次元力学場計測手法の開発

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  • OS0610 Study on the measurement of 3D mechanical field toward an elucidation of cell-ECM interaction

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Mechanical interactions between cells and extracellular matrix (ECM) direct many important cellular processes such as proliferation, migration and differentiation. It has been studied mostly about cell-ECM mechanical interaction in two dimensions, but enough knowledge was not obtained in three dimensions. It has been reported to be able to control the cell function by changing the elastic modulus of polyacrylamide gel. But many of its mechanism remain unknown. As a key to elucidate them, 3D mechanical interaction has been required. Therefore digital volume correlation (DVC), which is a method of measuring 3D displacement and strain fields within an object by using a pair of 3D volume images, is expected to be able to become a novel tool. In this paper, mechanical fields around hMSC cultured on collagen coated polyacrylamide gel is visualized by using DVC method based on confocal laser scanning microscopy. As a result, it is confirmed that hMSC on gel interacts dynamically with their surrounding 3D microenvironment. By studying the effects of mechanical fields on cell function by applying this analysis tool, we would be able to understand many physiological mechanisms clearly.

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