Minor contribution of cytotoxic T lymphocyte antigen 4 and programmed cell death 1 ligand 1 in immune tolerance against mouse thyrotropin receptor in mice
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- Yasui Junichi
- Department of Molecular Medicine, Atomic Bomb Disease Institute, Nagasaki University Department of Endocrinology and Metabolism, Nagasaki University Graduate School of Biomedical Sciences
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- Nakahara Mami
- Department of Molecular Medicine, Atomic Bomb Disease Institute, Nagasaki University
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- Shimamura Mika
- Department of Molecular Medicine, Atomic Bomb Disease Institute, Nagasaki University
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- Kurashige Tomomi
- Department of Molecular Medicine, Atomic Bomb Disease Institute, Nagasaki University
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- Yasui Kazuaki
- Department of Molecular Medicine, Atomic Bomb Disease Institute, Nagasaki University
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- Abiru Norio
- Department of Endocrinology and Metabolism, Nagasaki University Graduate School of Biomedical Sciences
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- Kawakami Atsushi
- Department of Endocrinology and Metabolism, Nagasaki University Graduate School of Biomedical Sciences
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- Nagayama Yuji
- Department of Molecular Medicine, Atomic Bomb Disease Institute, Nagasaki University
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抄録
We have previously shown that wild type (wt) mice exhibit susceptibility to immunization with human (h) thyrotropin receptor (TSHR), but resistance to mouse (m) TSHR, while TSHR knockout (KO) mice are susceptible to mTSHR, indicating the existence of robust immune tolerance against the mTSHR in wt mice. This tolerance may be mediated by either centrally or peripherally. We here explored the contribution of a peripheral arm of immune tolerance against the mTSHR by using antibodies to deplete regulatory T cells (Tregs), to antagonize co-inhibitory molecules and/or to stimulate co-stimulatory molecules. Antagonistic anti-co-inhibitory molecules, cytotoxic T lymphocyte antigen 4 (CTLA4) and programmed cell death 1 ligand 1 (PDL1), induced only low levels of anti-TSHR antibodies without induction of hyperthyroidism in a mouse Graves’ model. In this experimental setting, antibody levels were significantly higher in THSR+/- mice than wt mice. However, agonistic anti-co-stimulatory molecules, CD40 and CD137, and Treg-depleting anti-CD25 antibodies showed no effect. All these data suggest that peripheral immune tolerance against the mTSHR may play a minor role, and imply the importance of central tolerance, in immune tolerance against mTSHR in mice. Additional studies on central tolerance to the mTSHR will be necessary for completely delineating the mechanisms for immune tolerance against mTSHR in mice.
収録刊行物
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- Acta Medica Nagasakiensia
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Acta Medica Nagasakiensia 59 (1), 13-17, 2014
長崎大学医学部
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詳細情報 詳細情報について
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- CRID
- 1390001204675051264
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- NII論文ID
- 110009959607
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- NII書誌ID
- AA00508430
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- HANDLE
- 10069/34696
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- ISSN
- 00016055
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可