Two-year survival of Tricholoma matsutake ectomycorrhizas on Pinus densiflora seedlings after outplanting to a pine forest

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  • KOBAYASHI Hisayasu
    Ibaraki Prefectural Forestry Research Institute
  • TERASAKI Masataka
    Ibaraki Prefectural Forestry Research Institute:(Present office)Ibaraki Prefectural Government Office, Forest Policies Division
  • YAMADA Akiyoshi
    Ibaraki Prefectural Forestry Research Institute:Faculty of Agriculture, Shinshu University

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  • マツ林に野外植栽したマツ苗におけるマツタケ外生菌根の2年間の生存

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Abstract

In field trials of in vitro-generated pine ectomycorrhizal seedlings associated with Tricholoma matsutake, we examined the survivability of the ectomycorrhizas on Pinus densiflora seedlings one to three years after outplanting. In total, 55 seedlings (1 to 2 years old) were transplanted to two different P. densiflora experimental forests in Ibaraki Prefecture, Japan, from 2004 to 2008. One to three years after transplantation, the root systems of the seedlings were examined microscopically and their ectomycorrhizal tips were tested for the presence of fungus. T. matsutake was isolated from five seedlings one year after transplantation, and the identity of the fungal species was confirmed by PCR-RFLP analysis of the cultured mycelia. The same analysis showed that T. matsutake survived on one seedling two years after transplantation. These results suggest that T. matsutake ectomycorrhizas synthesized in vitro can survive at least two years after transplantation into a natural pine forest.

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