2H32 力学的負荷にともなう骨細胞動態解析に向けた組織内細胞形態の定量化

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  • 藤井 宣成
    京都大学大学院工学研究科マイクロエンジニアリング専攻
  • 須長 純子
    京都大学再生医科学研究所バイオメカニクス研究領域
  • 亀尾 佳貴
    京都大学再生医科学研究所バイオメカニクス研究領域
  • 上岡 寛
  • 安達 泰治
    京都大学再生医科学研究所バイオメカニクス研究領域

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  • 2H32 Quantification of osteocytic morphology within tissue under mechanical stress

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Osteocytes are believed to act as mechanosensory cells in mechanically adaptive bone remodeling. The cell body of osteocytes has various morphology depending on the types of bone, surrounding mechanical environment and degree of maturation. The difference in osteocyte morphology can significantly affect the mechanosensitivity of the cells. In this study, we aim at identifying the relationship between the maturation and morphology of osteocytes within tissue by the morphometry of osteocytes based on technique of fluorescence imaging. Using a mouse femur, we labeled the newly formed bone by calcein to visualize the maturation stage of osteocytes. Furthermore, we fluorescently stained actin cytoskeleton in osteocytes and measured aspect ratio of individual cell body. As a result, calcein-labeled bone clearly showed the process of bone formation, therefore enabled us to estimate the degree of osteocyte maturation. The aspect ratio of the osteocytes cell body did not depend on the degree of maturation. This result suggested that the morphology of osteocytes can be influenced by the mechanical environment when they are buried in osteoid during the differentiation, rather than the degree of maturation.

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