倒立型微分干渉顕微鏡に搭載した原子間力顕微鏡を精度100nmで位置制御しスライドガラス上の生物試料を観察するためのステージ  [in Japanese] Toritsugata bibun kansho kembikyo ni tosai sita genshiryoku kamryoku kenbikyo o seido 100nm de ichi seigyoshi suraido garasu jo no seibutsu shiryo o kansatsu suru tameno suteji  [in Japanese]

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Abstract

The atomic force microscope(AFM)has proved to be a useful tool to obtain geometrical information of the surface of objects at better than nanometer spatial resolution. When imaging by AFM the micron-sized biological objects deposited on a transparent substrate such as glass coverslip, we encounter the problem that the cantilever tip must be positioned over that transparent object with sub-micrometer positional control. It is quite difficult to visualize that transparent object with the optical microscope built in or externally linked to the AFM head. In this study a stand-alone-type AFM head was mounted on an inverted Nomarski/DIC optical microscope, which allowed transparent objects to be imaged together with the tip almost contacting the specimen surface. An X-Y stage, that can control the specimen position with X-and Y-pulse motors at 100-nm precision with respect to the cantilever tip, was integrated into this inverted microscope. The position of the cantilever tip, which is seen in the objective field of the optical microscope, is adjustable in both X-and Y-directions manually with micrometer screws independent of the specimen X-Y stage. This stage setting allowed high resolution imaging, e. g., of a sample of two dimensional protein arrays prepared on the surface of a glass coverslip, indicating that our AFM/Nomarski system enables simultaneous imaging at both micro-and nanometer spatial resolutions.

Journal

  • The Hiyoshi review of natural science

    The Hiyoshi review of natural science (36), 57-62, 2004

    慶應義塾大学日吉紀要刊行委員会

Codes

  • NII Article ID (NAID)
    120000804752
  • NII NACSIS-CAT ID (NCID)
    AN10079809
  • Text Lang
    JPN
  • Article Type
    departmental bulletin paper
  • Journal Type
    大学紀要
  • ISSN
    09117237
  • NDL Article ID
    7116911
  • NDL Source Classification
    ZM2(科学技術--科学技術一般--大学・研究所・学会紀要)
  • NDL Call No.
    Z14-1207
  • Data Source
    NDL  IR 
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