Expansion of donor-derived hematopoietic stem cells with PIGA mutation associated with late graft failure after allogeneic stem cell transplantation.

• Mochizuki, Kanako

• Sugimori, Chiharu

• Qi, Zhirong

• Lu, Xuzhang

• Takami, Akiyoshi

• Ishiyama, Ken

• Kondo, Yukio

• Yamazaki, Hirohito

• Okumura, Hirokazu

• Nakao, Shinji

Abstract

金沢大学附属病院血液内科

A small population of CD55(-)CD59(-) blood cells was detected in a patient who developed donor-type late graft failure after allogeneic stem cell transplantation (SCT) for treatment of aplastic anemia (AA). Chimerism and PIGA gene analyses showed the paroxysmal nocturnal hemoglobinuria (PNH)-type granulocytes to be of a donor-derived stem cell with a thymine insertion in PIGA exon 2. A sensitive mutation-specific polymerase chain reaction (PCR)-based analysis detected the mutation exclusively in DNA derived from the donor bone marrow (BM) cells. The patient responded to immunosuppressive therapy and achieved transfusion independence. The small population of PNH-type cells was undetectable in any of the 50 SCT recipients showing stable engraftment. The de novo development of donor cell-derived AA with a small population of PNH-type cells in this patient supports the concept that glycosyl phosphatidylinositol-anchored protein-deficient stem cells have a survival advantage in the setting of immune-mediated BM injury.

Journal

• Blood

  Blood 112 (5), 2160-2162, 2008-09-01

  American Society of Hematology

Details

• CRID: 1050282810960686976
• NII Article ID: 120000807462
• NII Article ID: 120001130400
• NII Book ID: AA12037546
• ISSN: 15280020
• ISSN: 00064971
• Web Site: http://hdl.handle.net/2297/12164
• Web Site: http://hdl.handle.net/2297/11731
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles