GABA-containing sympathetic preganglionic neurons in rat thoracic spinal cord send their axons to the superior cervical ganglion.

• ITO Tetsufumi

• HIOKI Hiroyuki

• NAKAMURA Kouichi

• TANAKA Yasuyo

• NAKADE Hiroyuki

• KANEKO Takeshi

• IINO Satoshi

• NOJYO Yoshiaki

抄録

GABA-containing fibers have been observed in the rat superior cervical ganglion (SCG) and, to a lesser extent, in the stellate ganglion (STG). The aim of present study is to clarify the source of these fibers. No cell body showed mRNAs for glutamic acid decarboxylases (GADs) or immunoreactivity for GAD of 67 kDa (GAD67) in the cervical sympathetic chain. Thus, GABA-containing fibers in the ganglia are suggested to be of extraganglionic origin. Since GAD67-immunoreactive fibers were found not in the dorsal roots or ganglia, but in the ventral roots, GABA-containing fibers in the sympathetic ganglia were considered to originate from the spinal cord. Furthermore, almost all GAD67-immunoreactive fibers in the sympathetic ganglia showed immunoreactivity for vesicular acetylcholine transporter, suggesting that GABA was utilized by some cholinergic preganglionic neurons. This was confirmed by the following results: 1) after injection of Sindbis/palGFP virus into the intermediolateral nucleus, some anterogradely labeled fibers in the SCG were immunopositive for GAD67, and 2) after injection of fluorogold into the SCG, some retrogradely labeled neurons in the thoracic spinal cord were positive for GAD67 mRNA. Finally, when the ventral roots of the eighth cervical to the fourth thoracic segments were cut, almost all GAD67- and GABA-immunoreactive fibers disappeared from the ipsilateral SCG and STG, suggesting that the vast majority of GABA-containing fibers in those ganglia were of spinal origin. Thus, the present findings strongly indicate that some sympathetic preganglionic neurons are not only cholinergic, but also GABAegic

収録刊行物

• The Journal of comparative neurology

  The Journal of comparative neurology 502 (1), 113-125, 2007-05

  John Wiley & Sons

キーワード

• nerve transection
• colocalization
• anterograde tracing
• retrograde tracing
• immunohistochemistry
• in situ hybridization

詳細情報

• CRID: 1571135652493349504
• NII論文ID: 120000807637
• ISSN: 00219967
• Web Site: http://hdl.handle.net/10098/1040
• 本文言語コード: en
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