Freeze-dried platelets promote hepatocyte proliferation in mice Freeze-dried platelets promote hepatocyte proliferation in mice

Access this Article

Search this Article

Abstract

In recent years, platelets are reported to promote liver, as well as bone regeneration and dermal wound healing. Platelets are required not only for thrombocytopenia treating but also for regenerative therapy. Platelets cannot be stored beyond three days, therefore, shortage of fresh platelets sometimes occurs. To preserve platelets for a long duration without degrading growth factors, a freeze-dried technique is required. We report here that platelets can be preserved by freeze-drying, using a programmed freezing method to avoid intracellular ice crystal formation. Freeze-dried platelets kept their morphological countenance and response with the agonist of thrombin was well maintained. Freeze-dried platelets stored adenine nucleotides, PDGF, and IGF-1 the same as those of fresh platelets. Freeze dried platelets also preserved their proliferative effect on hepatocytes identical to that of fresh platelets. These results of our study suggest that freeze dried platelets will obviate the storage problem of fresh platelets.

Journal

  • Cryobiology

    Cryobiology 55(3), 255-260, 2007-12

    Elsevier

Cited by:  3

Keywords

Codes

  • NII Article ID (NAID)
    120000835287
  • NII NACSIS-CAT ID (NCID)
    AA11544946
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    0011-2240
  • Data Source
    CJPref  IR 
Page Top