Regulation of c-myc through intranuclear localization of its RNA subspecies

• Shimizu, Noriaki

• Kawamoto, June-ko

• Utani, Koh-ichi

抄録

We used fluorescence in situ hybridization (FISH) to detect c-myc RNA subspecies in human COLO 320DM tumor cells. Although the FISH procedure removed the majority of RNAs from the nucleolus, c-myc RNA continued to be detected in both the nucleoplasm and nucleolus. This finding suggests stable association between c-myc RNA and the nucleolus. Nucleolar accumulation of c-myc RNA appeared to be temporally regulated by cell cycle progression. Hybridization with exon- and strand-specific RNA probes indicated that the non-protein coding exon 1 plays a novel role in determining the subnuclear localization of c-myc RNA. Antisense RNA targeting exon 2 localized only with nucleoplasmic foci, where it might interact with the sense strand. Thus, c-myc gene expression may be regulated by intranuclear localization of its RNA.

収録刊行物

• Biochemical and Biophysical Research Communications

  Biochemical and Biophysical Research Communications 359 (3), 806-810, 2007-08-03

  Academic Press Inc Elsevier Science

キーワード

• nucleolus
• RNA FISH
• nuclear structure and function
• post-transcriptional gene regulation
• c-myc

詳細情報

• CRID: 1050296265987133568
• NII論文ID: 120000875282
• NII書誌ID: AA00564395
• Web Site: http://ir.lib.hiroshima-u.ac.jp/00020744
• 本文言語コード: en
• 資料種別: journal article
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  • CiNii Articles
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