Elongation/compaction of giant DNA caused by depletion interaction with a flexible polymer

• Kojima, Masahiro

• Kubo, K.

• Yoshikawa, Kenichi

抄録

Structural changes in giant DNA induced by the addition of the flexible polymer Polyethylene Glycol (PEG) were examined by the method of single-DNA observation. In dilute DNA conditions, individual DNA assumes a compact state via a discrete coil-globule transition, whereas in concentrated solution, DNA molecules exhibit an extended conformation via macroscopic phase segregation. The long-axis length of the stretched state in DNA is about 10(3) times larger than that of the compact state. Phase segregation at high DNA concentrations occurs at lower PEG concentrations than the compaction at low DNA concentrations. These opposite changes in the conformation of DNA molecule are interpreted in terms of the free energy, including depletion interaction. (c) 2006 American Institute of Physics.

収録刊行物

• Journal of Chemical Physics

  Journal of Chemical Physics 124 (2), 2006-01-14

  American Institute of Physics

キーワード

• FOLDING TRANSITION
• CHAIN
• STRESS
• MATTER
• STATE

詳細情報

• CRID: 1050845760455790080
• NII論文ID: 120000896058
• NII書誌ID: AA00694991
• ISSN: 00219606
• HANDLE: 2433/45685
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles
  • KAKEN