Structural characterization of N-lignoceroyl (C24:0) sphingomyelin bilayer membranes : A reevaluation

• Takahashi, Hiroshi

• Hayakawa, Tomohiro

• Kawasaki, Yuko

• Ito, Kazuki

• Fujisawa, Tetsuro

• Kodama, Michiko

• Kobayashi, Toshihide

抄録

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Journal Article

Sphingomyelin (SM) is a membrane lipid and plays important roles in signaling, protein trafficking, cell growth and death. We investigated the structure of hydrated highly asymmetric SM, N-Lignoceroyl (C24:0) SM, bilayers with X-ray diffraction (XRD), simultanous small angle X-ray scattering (SAXS) and wide angle XRD, and SAXS measurements. At temperatures between two endothermic transitions of hydrated C24:0 SM bilayers, the C24:0 SM formed a ripple phase with the ripple periodicity of ~12-14 nm. About 3 month incubation at 277 K induced the formation of a stable phase with a short lamellar spacing of 5.62 nm. Based upon the structures revealed by this study and the phase behavior, we discuss the intermolecular interactions between C24:0 SM molecules in the bilayer membrane.

収録刊行物

• Journal of Applied Crystallography

  Journal of Applied Crystallography 40 s312-s317, 2007

  Munksgaard

キーワード

• SAXS
• stable phase
• ripple phase
• electron density profile
• XRD
• lamellar structure

詳細情報

• CRID: 1050001337662647936
• NII論文ID: 120000908540
• NII書誌ID: AA00693423
• ISSN: 00218898
• HANDLE: 10087/3153
• Web Site: https://gunma-u.repo.nii.ac.jp/records/2970
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles