Structure and thermal history dependant phase behavior of hydrated synthetic sphingomyelin analogue: 1,2-dimyristamido-1,2-deoxyphosphatidylcholine
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The physical properties of hydrated multilamellar sample of 1,2-dimyristamido-1,2-deoxyphosphatidylcholine (DDPC) were investigated by means of differential scanning calorimetry (DSC), static X-ray diffraction, and simultaneous DSC and X-ray diffraction. The DDPC is a synthetic sphingomyelin analogue and has two amide bonds in its hydrophobic parts. This paper reports on metastable phase behavior of the hydrated DDPC sample. By cooling from a chain-melted state at the rates of greater than 4 oC min-1, hydrated DDPC bilayers form a metastable gel phase. In the gel phase, the hydrophobic chains are tilted with respect to the bilayer normal, as like the gel phase of glycero-phosphatidylcholines. By heating, the metastable gel phase is transformed in to a stable phase associated with an exothermic heat event at 18.3 oC (DH = 14.6 kJ mol-1) and then the stable phase is transformed into a liquid-crystalline phase at 25.6 oC (DH = 42 kJ mol-1). The incubation at 17 oC for more than 1 hour also induces the formation of the stable phase. In the stable phase, the hydrophobic chains are packed into highly ordered crystal-like structure. However, the X-ray diffraction pattern of the stable phase suggested that the entire DDPC molecules do not form a two-dimensional molecular ordered lattice, differing from normal subgel phase of glycero-phosphatidylcholines. The structure and phase behavior of DDPC revealed by the present study are discussed from the viewpoint of hydrogen bonds.
収録刊行物
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- Biochimica et biophysica acta. Biomembranes
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Biochimica et biophysica acta. Biomembranes 1713 40-50, 2005-06
Elsevier
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詳細情報 詳細情報について
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- CRID
- 1050564287616070784
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- NII論文ID
- 120000908547
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- NII書誌ID
- AA00564657
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- ISSN
- 00052736
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- HANDLE
- 10087/3157
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- IRDB
- CiNii Articles