ヒラメParalichthys olivaceusのリンホシスチス細胞の微細構造

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  • Ultrastructures of lymphocystis cells in Japanese flounder Paralichthys olivaceus

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養殖ヒラメに発生したリンホシスチス細胞(LCC)の微細構造を観察した。若いLCCは既に厚い硝子状被膜を持って肥大しており,細胞質にはウイルス合成の場(AS),粗面小胞体,リボゾーム,ミトコンドリアがよく発達し,細胞質の樹状の突起が被膜内に伸張していた。 ASは顆粒状のinclusion zoneと基質部から構成され,inclusion zone表面でウイルス粒子が合成されていた。大きく肥大したLCCの核周囲の細胞質内ではASが崩壊し,ウイルス粒子(250-300nm)は結晶配列を示していた。細胞辺縁部では,多数のウイルス粒子を含むASは明瞭であるが,微小器官の多くは変性していた。

Histological signs and ultrastructures were studied on young and grown lymphocystis cells (LCCs) occurring in farmed Japanese flounder Paralichthys olivaceus. Young LCCs had a basophilic cytoplasm containing highly basophilic reticulations of inclusion bodies and a hypertrophied nucleus including prominent nucleoli, and were encapsulated with a thick hyaline wall. The cytoplasm extended many protrusions into the wall and was abundant in viral assembly sites (AS), rough endoplasmic reticula (rER), ribosomes, smooth endoplasmic reticula and mitochondria. AS consisted of inclusion zones on which viral particles began to be assembled and the surrounding matrix containing various maturing stages of virions. Grown LCCs had an eosinophilic cytoplasm displaying marginally located inclusion bodies with a decreased number and size. The cytoplasm displayed enormously large numbers of virions (250 to 300 nm in edge to edge diameter) showing crystalline array, and degenerated rER and mitochondria within the inner regions. The marginal areas of the cytoplasm contained a decreased size of AS, degenerated organelles and vacuoles. The nuclear membranes and cytoplasmic protrusions were degenerated, indicating that these cells were mature or old. Some fibroblasts formed inclusion bodies indicating LCCs derived from fibroblasts.

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