An RNA biding protein, Y14 interacts with and modulates STAT3 activation

• Ohbayashi, Norihiko

• Taira, Naohisa

• Kawakami, Shiho

• Togi, Sumihito

• Sato, Noriko

• Ikeda, Osamu

• Kamitani, Shinya

• Muromoto, Ryuta

• Sekine, Yuichi

• Matsuda, Tadashi

書誌事項

タイトル別名

• Interactions between STAT3 and Y14

抄録

Signal transducer and activator of transcription 3 (STAT3), which mediates biological actions in many physiological processes, is activated by cytokines and growth factors via specific tyrosine phosphorylation, dimerization and nuclear translocation. To clarify the molecular mechanisms underlying the regulation of STAT3 activation, we performed yeast two-hybrid screening. We identified Y14, an RNA-binding protein, as a novel STAT3-binding partner. Y14 bound to STAT3 through the C-terminal region of STAT3 in vivo. Importantly, small-interfering RNA-mediated reduction of endogenous Y14 expression decreased IL-6-induced tyrosine-phosphorylation, nuclear accumulation and DNA binding activity of STAT3, as well as IL-6/STAT3-dependent gene expression. These results indicate that Y14 interacts with STAT3 and regulates the transcriptional activation of STAT3 by influencing the tyrosine-phosphorylation of STAT3.

収録刊行物

• Biochemical and Biophysical Research Communications

  Biochemical and Biophysical Research Communications 372 (3), 475-479, 2008-08-01

  Academic Press

キーワード

• cytokine
• STAT3
• Y14
• RNA-binding protein
• gene expression
• signal transduction

詳細情報

• CRID: 1050845763926497920
• NII論文ID: 120000952719
• NII書誌ID: AA00564395
• ISSN: 10902104
• ISSN: 0006291X
• HANDLE: 2115/33897
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
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