Synthesis and properties of 4'-ThioDNA: unexpected RNA-like behavior of 4'-ThioDNA.

• Inoue, Naonori

• Minakawa, Noriaki

• Matsuda, Akira

Abstract

The synthesis and properties of fully modified 4'-thioDNAs, oligonucleotides consisting of 2'-deoxy-4'-thionucleosides, were examined. In addition to the known literature properties (preferable hybridization with RNA and resistance to endonuclease hydrolysis), we also observed higher resistance of 4'-thioDNA to 3'-exonuclease cleavage. Furthermore, we found that fully modified 4'-thioDNAs behaved like RNA molecules in their hybridization properties and structural aspect, at least in the case of the 4'-thioDNA duplex. This observation was confirmed by experiments using groove binders, in which a 4'-thioDNA duplex interacts with an RNA major groove binder, lividomycin A, but not with DNA groove binders, to give an increase in its thermal stability. Since a 4'-thioDNA duplex competitively inhibited the hydrolysis of an RNA duplex by RNase V1, it was not only the physical properties but also this biological data suggested that a 4'-thioDNA duplex has an RNA-like structure.

Journal

• Nucleic Acids Research

  Nucleic Acids Research 34 (12), 3476-3483, 2006

  Oxford University Press

Details

• CRID: 1050564288942828544
• NII Article ID: 120000965196
• ISSN: 13624962
• ISSN: 03051048
• HANDLE: 2115/26387
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles