Establishment of a newly improved detection system for NF-κB activity

• Matsuda, Mayuko

• Tsukiyama, Tadasuke

• Bohgaki, Miyuki

• Nonomura, Katsuya

• Hatakeyama, Shigetsugu

Abstract

The transcription factor nuclear factor-κB (NF-κB) plays roles in apoptosis, inflammation and oncogenesis. It is important for biological and medical research to understand when proteins of interest are activated in cells, leading to the establishment of a luciferase/EGFP assay to monitor the activation of transcription factors. Here, we describe an improved reporter system for NF-κB, the NF-κB-activated transgene (NAT) system that can detect NF-κB signalling with high sensitivity and specificity. The NAT system consists of large copy numbers of NF-κB consensus sequence and a minimal promoter derived from the mouse interleukin-2 (IL-2) gene. Furthermore, we generated NAT systems with stable or unstable luciferase/EGFP proteins. Stable and unstable types of luciferase/EGFP are suitable for analyzing the accumulation of and the real-time activity of NF-κB signal, respectively. Our findings suggest that the NAT system is effective for in vivo imaging of NF-κB signalling using cells or animals.

Journal

• Immunology Letters

  Immunology Letters 109 (2), 175-181, 2007-04-15

  Elsevier B.V.

Keywords

• NF-κB
• Transcription
• Reporter system
• Luciferase
• EGFP

Details

• CRID: 1050564288941576064
• NII Article ID: 120000968115
• HANDLE: 2115/24260
• ISSN: 01652478
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles