Fertilization-induced changes in the microtubular architecture of the maize egg cell and zygote—an immunocytochemical approach adapted to single cells

• Hoshino, Yoichiro

• Scholten, Stefan

• von Wiegen, Petra

• Lörz, Horst

• Kranz, Erhard

Abstract

By using single cell micromanipulation techniques, we developed an immunocytochemical procedure to examine subcellular protein localization in isolated and cultured cells. Localization of microtubules was examined in isolated single egg cells and developing zygotes of maize with anti--tubulin antibodies. In egg cells, a few cortical microtubules were detected but well organized microtubules were rarely observed. In contrast, distinct cortical microtubules and strands of cytoplasmic microtubules radiating from the nucleus to the cell periphery were observed in developing zygotes. Solely cortical microtubules were observed in zygotes up to 7 h after in vitro fertilization. After this time, radiating microtubules additionally appeared, and persisted during zygote development. These results indicate early and pronounced fertilization-induced changes in microtubular organization in the fertilized egg cell of maize.

Journal

• Sexual Plant Reproduction

  Sexual Plant Reproduction 17 (2), 89-95, 2004-07

  Springer

Keywords

• Anti-α-tubulin antibody
• Maize
• Microtubule
• Zea mays
• Zygote development

Details

• CRID: 1050845763913797248
• NII Article ID: 120000969339
• ISSN: 14322145
• ISSN: 09340882
• HANDLE: 2115/18874
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles