Development of a device to stretch tissue-like materials and to measure their mechanical properties by scanning probe microscopy.

• Mizutani, Takeomi

• Haga, Hisashi

• Kawabata, Kazushige

抄録

We have developed a new stretch device to investigate the biomechanical responses to an external loading force on a tissue-like material consisting of cells and a collagen gel. Collagen gel, a typical matrix found abundantly in the connective tissue, was attached to an elastic chamber that was precoated with a thin layer of collagen. Madin–Darby canine kidney cells that were cultured on the collagen gel were stretched in a uniaxial direction via deformation of the elastic chamber. Changes in the morphology and stiffness of the tissue-like structure were measured before and after the stretch using wide-range scanning probe microscopy (WR-SPM). The change in cellular morphology was heterogeneous, and there was a twofold increase in the intercellular junction due to the stretch. In addition to the WR-SPM measurements, this device enables observation of the spatial distribution of cytoskeletal proteins such as vimentin and α-catenin using immunofluorescent microscopy. We concluded that the stretch device we have reported in this paper is useful for measuring the mechanical response of a tissue-like material over a range of cell sizes when exposed to an external loading force.

収録刊行物

• Acta Biomaterialia

  Acta Biomaterialia 3 (4), 485-493, 2007-07

  Elsevier Ltd.

キーワード

• Scanning probe microscopy
• Collagen gel
• Stiffness
• External loading force
• Epithelial cell

詳細情報

• CRID: 1050845763921711488
• NII論文ID: 120000971009
• HANDLE: 2115/28420
• ISSN: 17427061
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles