Mutations in Ran system affected telomere silencing in Saccharomyces cerevisiae

• Hayashi, Naoyuki

• Kobayashi, Masahiko

• Shimizu, Hiroko

• Yamamoto, Ken-ichi

• Murakami, Seishi

• Nishimoto, Takeharu

抄録

The Ran GTPase system regulates the direction and timing of several cellular events, such as nuclear-cytosolic transport, centrosome formation, and nuclear envelope assembly in telophase. To gain insight into the Ran system's involvement in chromatin formation, we investigated gene silencing at the telomere in several mutants of the budding yeast Saccharomyces cerevisiae, which had defects in genes involved in the Ran system. A mutation of the RanGAP gene, rna1-1, caused reduced silencing at the telomere, and partial disruption of the nuclear Ran binding factor, yrb2-Δ2, increased this silencing. The reduced telomere silencing in rna1-1 cells was suppressed by a high dosage of the SIR3 gene or the SIT4 gene. Furthermore, hyperphosphorylated Sir3 protein accumulated in the rna1-1 mutant. These results suggest that RanGAP is required for the heterochromatin structure at the telomere in budding yeast. © 2007 Elsevier Inc. All rights reserved.

収録刊行物

• Biochemical and Biophysical Research Communications

  Biochemical and Biophysical Research Communications 363 (3), 788-794, 2007-11-23

  Elsevier

キーワード

• Budding yeast
• Gsp
• Heterochromatin
• Ran
• Rn1
• Sir3
• Sit4
• Snf2
• Telomere silencing
• Yrb2

詳細情報

• CRID: 1050282810960797824
• NII論文ID: 120001138430
• NII書誌ID: AA00564395
• ISSN: 0006291X
• Web Site: http://hdl.handle.net/2297/7371
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
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