Enhanced antigen presentation and CTL activity by transduction of mature rather than immature dendritic cells with octaarginine-modified liposomes

• Homhuan, Atthachai

• Kogure, Kentaro

• Nakamura, Takashi

• Shastri, Nilabh

• Harashima, Hideyoshi

抄録

To improve uptake and cross-presentation of exogenous antigens (Ag) by dendritic cells (DCs), octaarginine-modified liposomes (R8-Lip) were used as a novel strategy for protein-Ag transduction. Immature DCs endocytose macromolecules efficiently. While mature DCs lose their ability to capture Ag, but have an increased capacity for T-cell activation. Thus Ag-transduction has been performed mostly in immature DCs. In the present study, R8-Lip were efficiently taken up by both immature and mature DCs. DCs transduced after maturation were highly efficient at cross-presentation of Ag and induced higher cytotoxic T-lymphocytes (CTL) activity than were DCs transduced before maturation. The mechanism of Ag presentation involved the escape of R8-Lip from endosomes to cytosol, which require the acidic environment. The Ag released was then processed by a proteasome-dependent pathway. This novel transduction approach is clinically applicable, easy to perform, and has more practical advantages than current protein transduction methods.

収録刊行物

• Journal of Controlled Release

  Journal of Controlled Release 136 (1), 79-85, 2009-05-21

  Elsevier B.V.

キーワード

• Dendritic cells
• Protein transduction
• Octaarginine-modified liposomes
• Antigen presentation
• Cytotoxic T lymphocytes

詳細情報

• CRID: 1050845763931099392
• NII論文ID: 120001390958
• HANDLE: 2115/38744
• ISSN: 01683659
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles