Calcium response in single osteocytes to locally applied mechanical stimulus: Differences in cell process and cell body

• 安達, 泰治

• Aonuma, Yuki

• Tanaka, Mototsugu

• Hojo, Masaki

• Takano-Yamamoto, Teruko

• Kamioka, Hiroshi

抄録

It is proposed that osteocytes embedded in the bone matrix have the ability to sense deformation and/or damage to the matrix and to feed these mechanical signals back to the adaptive bone remodeling process. When osteoblasts differentiate into osteocytes during the bone formation process, they change their morphology to a stellate form with many slender processes. This characteristic cell shape may underlie the differences in mechanosensitivity between the cell processes and cell body. To elucidate the mechanism of cellular response to mechanical stimulus in osteocytes, we investigated the site-dependent response to quantitatively controlled local mechanical stimulus in single osteocytes isolated from chick embryos, using the technique of calcium imaging. A mechanical stimulus was applied to a single osteocyte using a glass microneedle targeting a microparticle adhered to the cell membrane by modification with a monoclonal antibody OB7.3. Application of the local deformation induced calcium transients in the vicinity of the stimulated point and caused diffusive wave propagation of the calcium transient to the entire intracellular region. The rate of cell response to the stimulus was higher when applied to the cell processes than when applied to the cell body. In addition, a large deformation was necessary at the cell body to induce calcium transients, whereas a relatively small deformation was sufficient at the cell processes, suggesting that the mechanosensitivity of the cell processes was higher than that of the cell body. These results suggest that the cell shape with slender processes contributes to the site-dependent mechanosensitivity in osteocytes.

収録刊行物

• Journal of Biomechanics

  Journal of Biomechanics 42 (12), 1989-1995, 2009-08

  Elsevier

キーワード

• Bone cells
• Calcium signaling response
• Cell biomechanics
• Mechanical stimulus
• Mechanotransduction
• Osteocytes

詳細情報

• CRID: 1050001202063113728
• NII論文ID: 120001596913
• ISSN: 00219290
• HANDLE: 2433/85228
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles
  • KAKEN